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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IGF-I protein

Activity

2-14 ng/mL EC50

Specific Activity

> 3.00 x 102 IU/mg

Species Reactivity

human,mouse

Purity

>95%

Cat no : HZ-1322

Synonyms

H-IGF-1, IGF, IGF-I, IGF-IA, IGF-IB, IGF1A, IGFIa, Insulin like growth factor, insulin-like growth factor 1 (somatomedin C), Insulin-like growth factor I, insulin-like growth factor IB, M-IGF-1, Mechano growth factor, MGF, OTTHUMP00000195084, R-IGF-1, Somatomedin C, Somatomedin-C

Validation Data Gallery

  • Purity of recombinant human IGF-I was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human IGF-I was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human IGF-I (Cat no: HZ-1322) stimulates dose-dependent proliferation of the MCF-7 human breast cancer cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. MCF-7 cells were treated with increasing concentrations of recombinant IGF-I for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 2-14 ng/mL.

    Recombinant human IGF-I (Cat no: HZ-1322) stimulates dose-dependent proliferation of the MCF-7 human breast cancer cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. MCF-7 cells were treated with increasing concentrations of recombinant IGF-I for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 2-14 ng/mL.

  • Proteintech IGF-I (Cat no: HZ-1322) demonstrates equivalent fold induction and a 2-fold decrease in EC50 compared to leading competitors. Recombinant human IGF-I stimulates dose-dependent proliferation of the MCF-7 human breast cancer cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. MCF-7 cells were treated with increasing concentrations of recombinant IGF-I for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 2-14 ng/mL.

    Proteintech IGF-I (Cat no: HZ-1322) demonstrates equivalent fold induction and a 2-fold decrease in EC50 compared to leading competitors. Recombinant human IGF-I stimulates dose-dependent proliferation of the MCF-7 human breast cancer cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. MCF-7 cells were treated with increasing concentrations of recombinant IGF-I for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 2-14 ng/mL.

Technical Specifications

GeneID 3479
Species Human
Expression HEK293
Activity 2-14 ng/mL EC50
Specific Activity> 3.00 x 102 IU/mg
Purity >95%
Endotoxin<1 EU/µg
Accession NumberP05019
Molecular Mass 9-10 kDa reduced and non-reduced, monomer, non-glycosylated
Formulation 50mM Acetate pH 4.0
Species Reactivity human,mouse

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

The Insulin like growth factor-1, also known as Somatomedin-C is a growth factor which is structurally related to insulin and is an important regulator of growth and differentiation in various tissues and cell systems. Human IGF-1 is synthesized as two precursor isoforms with N- and alternate C-terminal propeptide. The two precursor isoforms are differentially expressed by various tissues. The proteolytic cleavage of the N- and C-terminal regions results in the mature IGF-1 protein which is identical between isoforms. IGF-1 binds to IGF-1 receptor and induces receptor autophosphorylation. This further phosphorylates Insulin receptor substrate -1 (IRS-1) and activates various downstream signaling pathways including the PI3-AKT, MAPK etc. (PMID: 17354613, 17113337, 29535161)

Synonyms

H-IGF-1, IGF, IGF-I, IGF-IA, IGF-IB, IGF1A, IGFIa, Insulin like growth factor, insulin-like growth factor 1 (somatomedin C), Insulin-like growth factor I, insulin-like growth factor IB, M-IGF-1, Mechano growth factor, MGF, OTTHUMP00000195084, R-IGF-1, Somatomedin C, Somatomedin-C

Publications

SpeciesTitle
mouse

Sci Adv

Varying mechanical forces drive sensory epithelium formation

Authors - Mingyu Xia
View Article

ACS Biomater Sci Eng

Electrospun PCL Nerve Conduit Filled with GelMA Gel for CNTF and IGF-1 Delivery in Promoting Sciatic Nerve Regeneration in Rat

Authors - Hailiang Xu
View Article