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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human FGF-19 protein

Activity

50-300 ng/mL EC50

Species Reactivity

human,mouse

Purity

>95%

Cat no : HZ-1330

Synonyms

FGF-19, FGF19, FGFJ, Fibroblast Growth Factor-19

Validation Data Gallery

  • Purity of recombinant human FGF-19 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human FGF-19 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human FGF-19 (HZ-1330) stimulates dose-dependent proliferation of the NIH/3T3 mouse fibroblast cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. NIH/3T3 cells were serum starved with 0.02% FBS during treatment with increasing concentrations of recombinant human FGF-19 for 72hrs in defined medium. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range from 50-300 ng/mL.

    Recombinant human FGF-19 (HZ-1330) stimulates dose-dependent proliferation of the NIH/3T3 mouse fibroblast cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. NIH/3T3 cells were serum starved with 0.02% FBS during treatment with increasing concentrations of recombinant human FGF-19 for 72hrs in defined medium. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range from 50-300 ng/mL.

  • HepG2 cells were serum starved overnight, and treated with FGF-19 (HZ-1330) for 30 min at indicated concentrations. Cells were harvested and lysed using RIPA buffer with inhibitors. Western blot was performed with Proteintech antibodies, phospho-ERK1/2 (Thr202/Tyr204) Polyclonal antibody (28733-1-AP) and ERK1/2 Polyclonal antibody (11257-1-AP).

    HepG2 cells were serum starved overnight, and treated with FGF-19 (HZ-1330) for 30 min at indicated concentrations. Cells were harvested and lysed using RIPA buffer with inhibitors. Western blot was performed with Proteintech antibodies, phospho-ERK1/2 (Thr202/Tyr204) Polyclonal antibody (28733-1-AP) and ERK1/2 Polyclonal antibody (11257-1-AP).

Technical Specifications

GeneID 9965
Species Human
Expression HEK293
Activity 50-300 ng/mL EC50
Purity >95%
Endotoxin<1 EU/μg
Accession NumberO95750
Molecular Mass 23 kDa reduced, 22 kDa non-reduced, monomer, non-glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

FGF-19 is a member of the endocrine subfamily of FGFs. It primarily binds and activates signaling pathways downstream of FGFR4 in the liver. Through downregulation of the CYP7A1 enzyme, FGF-19 can prevent overproduction of bile salts in the liver and contribute towards their homeostasis. It is also a regulator of glucose and lipid metabolism alongside FGF-21. FGF-19 can additionally stimulate hepatocyte proliferation and has been correlated with the progression of hepatocellular carcinoma when its signaling levels are increased. (PMID: 30191047, 21437243, 20660733).

Synonyms

FGF-19, FGF19, FGFJ, Fibroblast Growth Factor-19

Publications

SpeciesTitle
mouse

Cell Metab

Hyodeoxycholic acid alleviates non-alcoholic fatty liver disease through modulating the gut-liver axis

Authors - Junliang Kuang
View Article