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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using pan-PKC Polyclonal antibody (12919-1-AP)

    WB analysis using 12919-1-AP (same clone as 12919-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12919-1-AP (PKC Beta antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12919-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse thymus tissue using pan-PKC Polyclonal antibody (12919-1-AP)

    WB analysis of mouse thymus using 12919-1-AP (same clone as 12919-1-PBS)

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12919-1-AP (PKC beta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12919-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using pan-PKC Polyclonal antibody (12919-1-AP)

    WB analysis using 12919-1-AP (same clone as 12919-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12919-1-AP (PKC beta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12919-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using pan-PKC Polyclonal antibody (12919-1-AP)

    IP experiment of mouse brain using 12919-1-AP (same clone as 12919-1-PBS)

    IP result of anti-pan-PKC (IP:12919-1-AP, 4ug; Detection:12919-1-AP 1:2000) with mouse brain tissue lysate 2100 ug. This data was developed using the same antibody clone with 12919-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using pan-PKC Polyclonal antibody (12919-1-AP)

    IHC staining of human breast cancer using 12919-1-AP (same clone as 12919-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12919-1-AP (PKC beta antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12919-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using pan-PKC Polyclonal antibody (12919-1-AP)

    IHC staining of human colon using 12919-1-AP (same clone as 12919-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12919-1-AP (pan-PKC antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12919-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using pan-PKC Polyclonal antibody (12919-1-AP)

    IHC staining of human breast cancer using 12919-1-AP (same clone as 12919-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12919-1-AP (PKC beta antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12919-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of K-562 cells using pan-PKC Polyclonal antibody (12919-1-AP)

    IF Staining of K-562 using 12919-1-AP (same clone as 12919-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using pan-PKC antibody (12919-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 12919-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of zebrafish retina using pan-PKC Polyclonal antibody (12919-1-AP)

    IF Staining of zebrafish retina using 12919-1-AP (same clone as 12919-1-PBS)

    Immunofluorescence analysis of bipolar cells labelled with pan-PKC polyclonal antibody (12919-1-AP, green) on tissue sections of the zebrafish retina at 5 days post fertilisation. Dilution 1:300. Tissue fixed overnight in 4% PFA. Müller glia, red; DAPI, blue; HuC/D, magenta. Data generated by Natalia Jaroszynska in Professor Ryan MacDonald's lab, University College London, UK. This data was developed using the same antibody clone with 12919-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of K-562 cells using pan-PKC Polyclonal antibody (12919-1-AP)

    FC experiment of K-562 using 12919-1-AP (same clone as 12919-1-PBS)

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human PKC Beta (12919-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 12919-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

pan-PKC Polyclonal antibody, PBS Only

pan-PKC Polyclonal Antibody for WB, IHC, IF, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 12919-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zebrafish

Applications

WB, IHC, IF, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

PKC beta, PRKCB, EC:2.7.11.13, PKC B, PKCB

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12919-1-PBS targets pan-PKC in WB, IHC, IF, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

Tested Reactivity human, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3591

Product name: Recombinant human PRKCB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 342-598 aa of BC036472

Sequence: FNFLMVLGKGSFGKVMLSERKGTDELYAVKILKKDVVIQDDDVECTMVEKRVLALPGKPPFLTQLHSCFQTMDRLYFVMEYVNGGDLMYHIQQVGRFKEPHAVFYAAEIAIGLFFLQSKGIIYRDLKLDNVMLDSEGHIKIADFGMCKENIWDGVTTKTFCGTPDYIAPEIIAYQPYGKSVDWWAFGVLLYEMLAGQAPFEGEDEDELFQSIMEHNVAYPKSMSKEAVAICKGLMTKHPGKRLGCGPEGERDIKEHA

相同性解析による交差性が予測される生物種
Full Name protein kinase C, beta
Calculated molecular weight 673 aa, 77 kDa
Observed molecular weight 77 kDa
GenBank accession numberBC036472
Gene Symbol PRKCB
Gene ID (NCBI) 5579
RRIDAB_2237469
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP05771
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. Protein kinase Cs (PKCs) are signaling molecules that play key roles in many cellular processes, including secretion, gene expression, proliferation, and muscle contraction. The PKC family is broadly divided into three subgroups: classical, novel, and atypical PKCs. These subgroups differ in both protein sequences and mechanistic requirements for catalytic activity. PKC beta is one of the PKC family members. PKC beta has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. This antibody can recognize PKC beta, PKC alpha, PKC gamma, PKC epsilon and PKC delta.

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