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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AKT Polyclonal antibody (27809-1-AP)

    WB analysis using 27809-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27809-1-AP (AKT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using AKT Polyclonal antibody (27809-1-AP)

    WB analysis using 27809-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 27809-1-AP (AKT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using AKT Polyclonal antibody (27809-1-AP)

    IHC staining of mouse testis using 27809-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27809-1-AP (AKT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using AKT Polyclonal antibody (27809-1-AP)

    IHC staining of mouse testis using 27809-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27809-1-AP (AKT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using AKT Polyclonal antibody (27809-1-AP)

    IHC staining of human colon cancer using 27809-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27809-1-AP (AKT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using AKT Polyclonal antibody (27809-1-AP)

    IHC staining of human colon cancer using 27809-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27809-1-AP (AKT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

AKT Polyclonal antibody

AKT Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27809-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

AKT 1, AKT1, EC:2.7.11.1, PKB, PKB ALPHA

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, NIH/3T3 cells, HEK-293 cells, HeLa cells, Jurkat cells
Positive IHC detected inmouse testis tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27809-1-AP targets AKT in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag26642

Product name: Recombinant human AKT protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 293-405 aa of BC000479

Sequence: FGLCKEGIKDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFELILMEEIRFPRTLGPEAKSLLSGLLKKDPKQRLGGGSEDAKEIMQH

相同性解析による交差性が予測される生物種
Full Name v-akt murine thymoma viral oncogene homolog 1
Calculated molecular weight 56 kDa
Observed molecular weight 56-62 kDa
GenBank accession numberBC000479
Gene Symbol AKT1
Gene ID (NCBI) 207
RRIDAB_3742259
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP31749
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AKT (also known as protein kinase B, PKB) is a serine threonine protein kinase consisting of three isoforms, AKT1, AKT2, and AKT3, which are encoded by three distinct genes, PKBα, PKBβ, and PKBγ, respectively. Among them, AKT1 is ubiquitously expressed and primarily regulates cell survival, AKT2 controls glucose metabolism, while AKT3 is highly expressed in the brain and testes. These isoforms are central nodes in the PI3K-AKT-mTOR signaling pathway, which is a critical regulator of cell survival, growth, proliferation, and metabolism (PMID: 25811375, PMID: 31173856, PMID: 36180910). This antibody detects total AKT1, AKT2, and AKT3 isoforms, typically showing a molecular weight of approximately 56-62 kDa in Western blot analysis.

Protocols

Product Specific Protocols
IHC protocol for AKT antibody 27809-1-APDownload protocol
WB protocol for AKT antibody 27809-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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