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Host
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

  • TPI1 promoter: 8914-9 Start codon (ATG): 10-12 Spot-Tag®: 13-48 MCS: 53-112 T7 primer site: 230-248 Leucin marker (LEU2): 1564-2670 2µ replication origin: 3396-4865 Ampicillin resistance gene: 5723-6583 pRB322 replication origin: 6738-7357

    TPI1 promoter: 8914-9 Start codon (ATG): 10-12 Spot-Tag®: 13-48 MCS: 53-112 T7 primer site: 230-248 Leucin marker (LEU2): 1564-2670 2µ replication origin: 3396-4865 Ampicillin resistance gene: 5723-6583 pRB322 replication origin: 6738-7357

  • Multiple cloning site (MCS)

    Multiple cloning site (MCS)

ChromoTek pSpot8 vector

The plasmid pSpot8 is an expression vector for Spot-Tag® fusion proteins in S. cerevisiae. After cloning the protein of interest (POI) into the multiple cloning site (MCS) provided by pSpot8, the Spot-Tag® (sequence: PDRVRAVSHWSS) will be fused to the C-terminus of the POI.
Cat No. ev-8

Vector type

yeast expression vector

Promoter

TPI1

Reporter

Spot-Tag® C-terminal

Synonyms

pSpot8 vector, Spot vector

Size/Concentration: 

-/ -

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Product Information

The plasmid pSpot8 is an expression vector for Spot-Tag® fusion proteins in S. cerevisiae. After cloning the protein of interest (POI) into the multiple cloning site (MCS) provided by pSpot8, the Spot-Tag® (sequence: PDRVRAVSHWSS) will be fused to the C-terminus of the POI.

ApplicationsProtein expression
Vector typeyeast expression vector
PromoterTPI1
Replication2µ (S. cerevisiae) pBR322 (E. coli)
ReporterSpot-Tag® (PDRVRAVSHWSS) C-terminal
Selectionleucin (S. cerevisiae) ampicillin (E. coli)