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Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

  • TPI1 promoter: 9576-9 GFPuv-Spot-Tag®: 10-780 Spot-Tag®: 742-777 T7 primer site: 892-910 Leucin marker (LEU2): 2226-3332 2µ replication origin: 4058-5527 Ampicillin resistance gene: 6385-7245 pMB1 replication origin: 7400-8019

    TPI1 promoter: 9576-9 GFPuv-Spot-Tag®: 10-780 Spot-Tag®: 742-777 T7 primer site: 892-910 Leucin marker (LEU2): 2226-3332 2µ replication origin: 4058-5527 Ampicillin resistance gene: 6385-7245 pMB1 replication origin: 7400-8019

ChromoTek pSpot8 GFP-Spot-Tag

The plasmid pSpot8_GFP-Spot-Tag is a S. cerevisiae expression vector encoding the Aequorea victoria Green fluorescent protein (GFP) variant GFPuv fused to the Spot-Tag®. The Spot-Tag® comprises the sequence PDRVRAVSHWSS and is fused to the C-terminus of GFPuv. This plasmid may be used as a positive control for immunoprecipitation or Western blot experiments using the Spot-Tag® system.
Cat No. ev-33

Vector type

Yeast expression vector

Promoter

TPI1

Reporter

Spot-Tag® C-termina

Synonyms

Size/Concentration: 

-/ -

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Product Information

The plasmid pSpot8_GFP-Spot-Tag is a S. cerevisiae expression vector encoding the Aequorea victoria Green fluorescent protein (GFP) variant GFPuv fused to the Spot-Tag®. The Spot-Tag® comprises the sequence PDRVRAVSHWSS and is fused to the C-terminus of GFPuv. This plasmid may be used as a positive control for immunoprecipitation or Western blot experiments using the Spot-Tag® system.

DescriptionThe plasmid pSpot8_GFP-Spot-Tag is a S. cerevisiae expression vector encoding the Aequorea victoria Green fluorescent protein (GFP) variant GFPuv fused to the Spot-Tag®. The Spot-Tag® comprises the sequence PDRVRAVSHWSS and is fused to the C-terminus of GFPuv. This plasmid may be used as a positive control for immunoprecipitation or Western blot experiments using the Spot-Tag® system.
ApplicationsProtein expression
Vector typeYeast expression vector
PromoterTPI1
Replication2µ (S. cerevisiae) pBR322 (E. coli)
ReporterSpot-Tag® (PDRVRAVSHWSS) C-termina
SelectionLeucin (S. cerevisiae) Ampicillin (E. coli)