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Host
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

  • TPI1 promoter: 7232-9 MCS: 14-73 Spot-Tag®: 73-108 Stop codon (TAA): 109-111 T7 primer site: 223-241 Leucin marker (LEU2): 1557-2663 CEN replication origin: 3391-3909 Ampicillin resistance gene: 4041-4901 pRB322 replication origin: 5056-5675

    TPI1 promoter: 7232-9 MCS: 14-73 Spot-Tag®: 73-108 Stop codon (TAA): 109-111 T7 primer site: 223-241 Leucin marker (LEU2): 1557-2663 CEN replication origin: 3391-3909 Ampicillin resistance gene: 4041-4901 pRB322 replication origin: 5056-5675

  • Multiple cloning site (MCS)

    Multiple cloning site (MCS)

ChromoTek pSpot7 vector

The plasmid pSpot7 is an expression vector for Spot-Tag® fusion proteins in S. cerevisiae. After cloning the protein of interest (POI) into the multiple cloning site (MCS) provided by pSpot7, the Spot-Tag® (sequence: PDRVRAVSHWSS) will be fused to the N-terminus of the POI.
Cat No. ev-7

Vector type

yeast expression vector

Promoter

TPI1

Reporter

Spot-Tag® N-terminal

Synonyms

pSpot7 vector, Spot vector

Size/Concentration: 

-/ -

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Product Information

The plasmid pSpot7 is an expression vector for Spot-Tag® fusion proteins in S. cerevisiae. After cloning the protein of interest (POI) into the multiple cloning site (MCS) provided by pSpot7, the Spot-Tag® (sequence: PDRVRAVSHWSS) will be fused to the N-terminus of the POI.

ApplicationsProtein expression
Vector typeyeast expression vector
PromoterTPI1
Replication2µ (S. cerevisiae) pBR322 (E. coli)
ReporterSpot-Tag® (PDRVRAVSHWSS) N-terminal
Selectionleucin (S. cerevisiae) ampicillin (E. coli)

Documentation

SDS
ev-7_SDS_pSpot7 (EN)
Datasheet
ev-7.pdf