ChromoTek pSpot5 vector

The plasmid pSpot5 is an expression vector for Spot-Tag® fusion proteins in S. cerevisiae. After cloning the protein of interest (POI) into the multiple cloning site (MCS) provided by pSpot5, the Spot-Tag® (sequence: PDRVRAVSHWSS) will be fused to the N-terminus of the POI.

Vector type

yeast expression vector

Promoter

TPI1

Reporter

Spot-Tag® N-terminal

Cat no : ev-5

Synonyms

pSpot5 vector, Spot vector



Product Information

The plasmid pSpot5 is an expression vector for Spot-Tag® fusion proteins in S. cerevisiae. After cloning the protein of interest (POI) into the multiple cloning site (MCS) provided by pSpot5, the Spot-Tag® (sequence: PDRVRAVSHWSS) will be fused to the N-terminus of the POI.

ApplicationsProtein expression
Vector typeyeast expression vector
PromoterTPI1
ReplicationCEN (S. cerevisiae) pBR322 (E. coli)
ReporterSpot-Tag® (PDRVRAVSHWSS) N-terminal
Selectionleucin (S. cerevisiae) Ampicillin (E. coli)6

Documentation

SDS
SDS.pdf
Datasheet
ev-5.pdf

Publications

ApplicationTitle

Life Sci Alliance

The stabilized Pol31-Pol3 interface counteracts Pol32 ablation with differential effects on repair.

Authors - Kenji Shimada