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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

  • TPI1 promoter: 7239-9 Start codon (ATG): 10-12 Spot-Tag®: 13-48 MCS: 47-112 T7 primer site: 230-248 Leucin marker (LEU2): 1564-2670 CEN replication origin: 3398-3916 Ampicillin resistance gene: 4048-4908 pRB322 replication origin: 5063-5682

    TPI1 promoter: 7239-9 Start codon (ATG): 10-12 Spot-Tag®: 13-48 MCS: 47-112 T7 primer site: 230-248 Leucin marker (LEU2): 1564-2670 CEN replication origin: 3398-3916 Ampicillin resistance gene: 4048-4908 pRB322 replication origin: 5063-5682

  • Multiple cloning site (MCS)

    Multiple cloning site (MCS)

ChromoTek pSpot5 vector

The plasmid pSpot5 is an expression vector for Spot-Tag® fusion proteins in S. cerevisiae. After cloning the protein of interest (POI) into the multiple cloning site (MCS) provided by pSpot5, the Spot-Tag® (sequence: PDRVRAVSHWSS) will be fused to the N-terminus of the POI.
Cat No. ev-5
Publications(1)

Vector type

yeast expression vector

Promoter

TPI1

Reporter

Spot-Tag® N-terminal

Synonyms

pSpot5 vector, Spot vector

Size/Concentration: 

-/ -

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Product Information

The plasmid pSpot5 is an expression vector for Spot-Tag® fusion proteins in S. cerevisiae. After cloning the protein of interest (POI) into the multiple cloning site (MCS) provided by pSpot5, the Spot-Tag® (sequence: PDRVRAVSHWSS) will be fused to the N-terminus of the POI.

ApplicationsProtein expression
Vector typeyeast expression vector
PromoterTPI1
ReplicationCEN (S. cerevisiae) pBR322 (E. coli)
ReporterSpot-Tag® (PDRVRAVSHWSS) N-terminal
Selectionleucin (S. cerevisiae) Ampicillin (E. coli)6

Documentation

SDS
ev-5_SDS_pSpot5 (EN)
Datasheet
ev-5.pdf

Publications

ApplicationTitle

Life Sci Alliance

The stabilized Pol31-Pol3 interface counteracts Pol32 ablation with differential effects on repair.

Authors - Kenji Shimada
View Article