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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

  • HeLa cells were transfected with the Spot-Tag®-Actin plasmid overnight, fixed, stained with Spot-Label ATTO594 (product code eba594), and imaged using 40X objective. Spot-Tag®-Actin filaments in red, cell nuclei in blue (DAPI). Scale bar 10 µm.

    HeLa cells were transfected with the Spot-Tag®-Actin plasmid overnight, fixed, stained with Spot-Label ATTO594 (product code eba594), and imaged using 40X objective. Spot-Tag®-Actin filaments in red, cell nuclei in blue (DAPI). Scale bar 10 µm.

  • CMV promoter: 508-589 Spot-Tag®-actin: 613-1776 Spot-Tag®: 616-651 SV40 terminator: 1799-2043 Kanamycin resistance gene: 3005-3805 pMB1 replication origin: 4400-5019

    CMV promoter: 508-589 Spot-Tag®-actin: 613-1776 Spot-Tag®: 616-651 SV40 terminator: 1799-2043 Kanamycin resistance gene: 3005-3805 pMB1 replication origin: 4400-5019

ChromoTek pSpot-Tag-Actin vector

The plasmid pSpot-Tag®-Actin is a mammalian expression vector encoding human β-actin fused to the Spot-Tag®. The Spot-Tag® comprises the sequence PDRVRAVSHWSS and is fused to the N-terminus of β-actin. This plasmid may be used as a positive control for immunofluorescence microscopy or Western blot experiments using the Spot-Tag® system.
Cat No. ev-31

Vector type

Mammalian expression vector

Promoter

CMV

Reporter

Spot-Tag® N-terminal

Synonyms

pSpot-Tag-Actin vector, Spot vector

Size/Concentration: 

-/ -

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Product Information

The plasmid pSpot-Tag®-Actin is a mammalian expression vector encoding human β-actin fused to the Spot-Tag®. The Spot-Tag® comprises the sequence PDRVRAVSHWSS and is fused to the N-terminus of β-actin. This plasmid may be used as a positive control for immunofluorescence microscopy or Western blot experiments using the Spot-Tag® system.

DescriptionThe plasmid pSpot-Tag®-Actin is a mammalian expression vector encoding human beta-actin fused to the Spot-Tag®. The Spot-Tag® comprises the sequence PDRVRAVSHWSS and is fused to the N-terminus of β-actin. This plasmid may be used as a positive control for immunofluorescence microscopy or Western blot experiments using the Spot-Tag® system.
ApplicationsProtein expression1
Vector typeMammalian expression vector
PromoterCMV
ReplicationpMB1
ReporterSpot-Tag® (PDRVRAVSHWSS)N-terminal
Codon usageMammalian
SelectionKan/Neo