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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NGFR/TNFRSF16 Recombinant antibody (85192-1-RR)

    WB analysis using 85192-1-RR (same clone as 85192-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85192-1-RR (p75NTR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85192-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85192-1-RR

    Affinity and Kinetic Characterization of 85192-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85192-1-RR against Rat NGFR/TNFRSF16 were performed. The affinity constant is 0.148 nM.

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Rat NGFR/TNFRSF16 Recombinant monoclonal antibody, PBS Only

NGFR/TNFRSF16 Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 85192-1-PBS
Clone No.242465H4

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, Indirect ELISA

Synonyms

Low-affinity nerve growth factor receptor p75NGFR, Low-affinity nerve growth factor receptor, Low affinity neurotrophin receptor p75NTR, Gp80-LNGFR, CD271

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85192-1-PBS targets NGFR/TNFRSF16 in WB, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name nerve growth factor receptor (TNFR superfamily, member 16)
Calculated molecular weight45 kDa
Observed molecular weight70-75 kDa
Gene Symbol Ngfr
Gene ID (NCBI) 24596
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP07174
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

p75NTR, also named TNFRSF16, Gp80-LNGFR, p75 ICD, and CD271, is a low-affinity receptor that can bind to NGF, BDNF, NT-3, and NT-4. It mediates cell survival as well as cell death of neural cells. The antibody is specific to NGFR.