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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AMACR/p504S Monoclonal antibody (60240-1-Ig)

    WB analysis using 60240-1-Ig (same clone as 60240-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60240-1-Ig (p504S,AMACR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 60240-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using AMACR/p504S Monoclonal antibody (60240-1-Ig)

    WB analysis using 60240-1-Ig (same clone as 60240-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60240-1-Ig (p504S,AMACR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60240-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using AMACR/p504S Monoclonal antibody (60240-1-Ig)

    WB analysis of HEK-293 using 60240-1-Ig (same clone as 60240-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 60240-1-Ig (p504S,AMACR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60240-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using AMACR/p504S Monoclonal antibody (60240-1-Ig)

    IF Staining of A431 using 60240-1-Ig (same clone as 60240-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using p504S,AMACR antibody (60240-1-Ig, Clone: 2H9B5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60240-1-PBS in a different storage buffer formulation.

AMACR/p504S Monoclonal antibody, PBS Only

AMACR/p504S Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 60240-1-PBS
Clone No.2H9B5

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

AMACR, p504S, EC:5.1.99.4, Alpha-methylacyl-CoA racemase, 2-methylacyl-CoA racemase

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60240-1-PBS targets AMACR/p504S in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag8720

Product name: Recombinant human p504S,AMACR protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-135 aa of BC009471

Sequence: MALQGISVMELSGLAPGPFCAMVLADFGARVVRVDRPGSRYDVSRLGRGKRSLVLDLKQPRGAAVLRRLCKRSDVLLEPFRRGVMEKLQLGPEILQRENPRLIYARLSGFGQSGSFCRLAGHDINYLALSGGRNS

相同性解析による交差性が予測される生物種
Full Name alpha-methylacyl-CoA racemase
Calculated molecular weight 382 aa, 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC009471
Gene Symbol AMACR
Gene ID (NCBI) 23600
RRIDAB_2881363
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ9UHK6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

AMACR(Alpha-methyl acyl-CoA racemase) belongs to the CaiB/BaiF CoA-transferase family. It is a mitochondrial and peroxisomal enzyme that catalyzes the conversion of 2R stereoisomers of phytanic and pristanic acid to their S counterparts. AMACR has previously been shown to be a highly sensitive marker for colorectal and clinically localized prostate cancer (PCa). However, AMACR expression is down-regulated at the transcript and protein level in hormone-refractory metastatic PCa, suggesting a hormone-dependent expression of AMACR(PMID:12213712). It has 3 isoforms produced by alternative splicing. Defects in AMACR are the cause of alpha-methyl acyl-CoA racemase deficiency (AMACRD) and congenital bile acid synthesis defect type 4 (CBAS4).

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