Validation Data Gallery

Filter: AllWBIHC

WB analysis of Jurkat using 14064-1-AP
WB result of p38 antibody (14064-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-p38 transfected Jurkat cells.
WB analysis using 14064-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14064-1-AP (p38 MAPK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 14064-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14064-1-AP (p38 MAPK antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis using 14064-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14064-1-AP (p38 MAPK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of RAW 264.7 using 14064-1-AP
N/A
WB analysis using 14064-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14064-1-AP (p38 MAPK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 14064-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 14064-1-AP (p38 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human colon cancer using 14064-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14064-1-AP (p38 MAPK antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 14064-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14064-1-AP (p38 MAPK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 14064-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14064-1-AP (p38 MAPK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 14064-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14064-1-AP (p38 MAPK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 14064-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14064-1-AP (p38 MAPK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 14064-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14064-1-AP (p38 MAPK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 14064-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14064-1-AP (p38 MAPK antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

p38 MAPK Polyclonal antibody

p38 MAPK Polyclonal Antibody for WB, IHC, ELISA

Cat No. 14064-1-AP

Publications(846)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

WB, IHC, IF, ELISA

Synonyms

MAPK14, CSAID-binding protein, CSBP, CSBP1, CSBP2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (846)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, Jurkat cells, mouse heart tissue, RAW 264.7 cells, NIH/3T3 cells, rat spleen tissue, Neuro-2a cells, rat heart tissue, K-562 cells
Positive IHC detected in	human colon cancer tissue, human liver cancer tissue, mouse heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunohistochemistry (IHC)	IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 750 publications below
IHC	See 16 publications below
IF	See 18 publications below
ELISA	See 1 publications below

Product Information

14064-1-AP targets p38 MAPK in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, rabbit, chicken, zebrafish, goat, fish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5115 Product name: Recombinant human p38 MAPK protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-307 aa of BC031574 Sequence: MSQERPTFYRQELNKTIWEVPERYQNLSPVGSGAYGSVCAAFDTKTGLRVAVKKLSRPFQSIIHAKRTYRELRLLKHMKHENVIGLLDVFTPARSLEEFNDVYLVTHLMGADLNNIVKCQKLTDDHVQFLIYQILRGLKYIHSADIIHRDLKPSNLAVNEDCELKILDFGLARHTDDEMTGYVATRWYRAPEIMLNWMHYNQTVDIWSVGCIMAELLTGRTLFPGTDHIDQLKLILRLVGTPGAELLKKISSESARNYIQSLTQMPKMNFANVFIGANPLAVDLLEKMLVLDSDKRITAAQALAHAY 相同性解析による交差性が予測される生物種
Full Name	mitogen-activated protein kinase 14
Calculated molecular weight	360 aa, 41 kDa
Observed molecular weight	38-42 kDa
GenBank accession number	BC031574
Gene Symbol	p38 MAPK
Gene ID (NCBI)	1432
RRID	AB_2878007
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q16539
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MAPK14(mitogen-activated protein kinase 14) is also named as SAPK2A, p38MAPK, CSBP1, RK, p38, EXIP, Mxi2, CSBP2, PRKM14, PRKM15, CSPB1, p38ALPHA and belongs to the MAP kinase subfamily. MAPK14-signaling is a central pathway for the integration of instructive signals in dendritic cells for T(H)17 differentiation and inflammation(PMID:22231518). It plays an important role in the regulation of hematopoietic stem cellself-renewal in vitro and inhibition of MAPK14 activation with a small molecule inhibitor may represent a novel approach to promote ex vivo expansion of hematopoietic stem cell(PMID:21198398). This protein has some isoforms with MW 29-31 kDa, 35 kDa and 41 kDa.

Protocols

Product Specific Protocols
IHC protocol for p38 MAPK antibody 14064-1-AP	Download protocol
WB protocol for p38 MAPK antibody 14064-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Mol Cancer Targeting BATF2-RGS2 axis reduces T-cell exhaustion and restores anti-tumor immunity Authors - Xuyu Gu View Article
mouse	WB	Immunity Innate immune sensing of Z-nucleic acids by ZBP1-RIPK1 axis drives neuroinflammation in Alzheimer's disease. Authors - Ziwen Song View Article
human	WB	Cancer Discov The Meningioma Enhancer Landscape Delineates Novel Subgroups and Drives Druggable Dependencies. Authors - Briana C Prager View Article
mouse	WB,IHC,IF,ELISA	Adv Mater Ultrasound-Activated Sonophage Synergizes Sonodynamic Therapy and Saltoptosis for Solid Tumor Eradication. Authors - Kerong Chen View Article
rat	WB	Mil Med Res D-mannose alleviates intervertebral disc degeneration through glutamine metabolism Authors - Zheng-Lin Dong View Article
mouse	WB	Cancer Commun (Lond) Reciprocal tumor-platelet interaction through the EPHB1-EFNB1 axis in the liver metastatic niche promotes metastatic tumor outgrowth in pancreatic ductal adenocarcinoma Authors - Lin-Li Yao View Article

Validation Data Gallery

WB analysis of Jurkat using 14064-1-AP

WB analysis using 14064-1-AP

WB analysis using 14064-1-AP

WB analysis using 14064-1-AP

WB analysis of RAW 264.7 using 14064-1-AP

WB analysis using 14064-1-AP

WB analysis of mouse heart using 14064-1-AP

IHC staining of human colon cancer using 14064-1-AP

IHC staining of human colon cancer using 14064-1-AP

IHC staining of human colon cancer using 14064-1-AP

IHC staining of human liver cancer using 14064-1-AP

IHC staining of human liver cancer using 14064-1-AP

IHC staining of human liver cancer using 14064-1-AP

IHC staining of mouse heart using 14064-1-AP

p38 MAPK Polyclonal antibody

p38 MAPK Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Targeting BATF2-RGS2 axis reduces T-cell exhaustion and restores anti-tumor immunity

Innate immune sensing of Z-nucleic acids by ZBP1-RIPK1 axis drives neuroinflammation in Alzheimer's disease.

The Meningioma Enhancer Landscape Delineates Novel Subgroups and Drives Druggable Dependencies.

Ultrasound-Activated Sonophage Synergizes Sonodynamic Therapy and Saltoptosis for Solid Tumor Eradication.

D-mannose alleviates intervertebral disc degeneration through glutamine metabolism

Reciprocal tumor-platelet interaction through the EPHB1-EFNB1 axis in the liver metastatic niche promotes metastatic tumor outgrowth in pancreatic ductal adenocarcinoma

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