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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using p19 INK4d Polyclonal antibody (10272-2-AP)

    WB analysis using 10272-2-AP (same clone as 10272-2-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 10272-2-AP (p19 INK4d antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10272-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using p19 INK4d Polyclonal antibody (10272-2-AP)

    IHC staining of mouse testis using 10272-2-AP (same clone as 10272-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10272-2-AP (p19 INK4d antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10272-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using p19 INK4d Polyclonal antibody (10272-2-AP)

    IHC staining of mouse testis using 10272-2-AP (same clone as 10272-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10272-2-AP (p19 INK4d antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10272-2-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

p19 INK4d Polyclonal antibody, PBS Only

p19 INK4d Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 10272-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10272-2-PBS targets p19 INK4d in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0415

Product name: Recombinant human P19 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-166 aa of BC001822

Sequence: MLLEEVRAGDRLSGAAARGDVQEVRRLLHRELVHPDALNRFGKTALQVMMFGSTAIALELLKQGASPNVQDTSGTSPVHDAARTGFLDTLKVLVEHGADVNVPDGTGALPIHLAVQEGHTAVVSFLAAESDLHRRDARGLTPLELALQRGAQDLVDILQGHMVAPL

相同性解析による交差性が予測される生物種
Full Name cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4)
Calculated molecular weight 19 kDa
Observed molecular weight 18-20 kDa
GenBank accession numberBC001822
Gene Symbol p19 INK4d/CDKN2D
Gene ID (NCBI) 1032
RRIDAB_2078742
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP55273
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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