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Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using DCTN1/p150 glued Polyclonal antibody (55182-1-AP)

    WB analysis of human brain using 55182-1-AP (same clone as 55182-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 55182-1-AP (DCTN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55182-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using DCTN1/p150 glued Polyclonal antibody (55182-1-AP)

    WB analysis of HeLa using 55182-1-AP (same clone as 55182-1-PBS)

    WB result of DCTN1/p150 glued antibody (55182-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-DCTN1/p150 glued transfected HeLa cells. This data was developed using the same antibody clone with 55182-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using DCTN1/p150 glued Polyclonal antibody (55182-1-AP)

    WB analysis of Jurkat using 55182-1-AP (same clone as 55182-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 55182-1-AP (DCTN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55182-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using DCTN1/p150 glued Polyclonal antibody (55182-1-AP)

    IHC staining of human brain using 55182-1-AP (same clone as 55182-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 55182-1-AP (DCTN1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55182-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using DCTN1/p150 glued Polyclonal antibody (55182-1-AP)

    IHC staining of human brain using 55182-1-AP (same clone as 55182-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 55182-1-AP (DCTN1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55182-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using DCTN1/p150 glued Polyclonal antibody (55182-1-AP)

    IF Staining of MDCK using 55182-1-AP (same clone as 55182-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MDCK cells using 55182-1-AP (p150 glued antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55182-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

DCTN1/p150 glued Polyclonal antibody, PBS Only

DCTN1/p150 glued Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 55182-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, canine

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

DCTN1, p150 glued, DP-150, DCTN 1, DAP-150

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

55182-1-PBS targets DCTN1/p150 glued in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, canine samples.

Tested Reactivity human, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name dynactin 1 (p150, glued homolog, Drosophila)
Calculated molecular weight 142 kDa
Observed molecular weight 135 kDa, 150 kDa
GenBank accession numberNM_004082
Gene Symbol p150 glued
Gene ID (NCBI) 1639
RRIDAB_10837074
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14203
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DCTN1, also named as DAP-150, p150-glued and p135, belongs to the dynactin 150 kDa subunit family. It is required for the cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein-dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles. Defects in DCTN1 are the cause of distal hereditary motor neuronopathy type 7B (HMN7B). This antibody is specific to DCTN1.