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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using p130Cas / BCAR1 Monoclonal antibody (67215-1-Ig)

    WB analysis using 67215-1-Ig (same clone as 67215-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67215-1-Ig (p130Cas / BCAR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using p130Cas / BCAR1 Monoclonal antibody (67215-1-Ig)

    IHC staining of human breast cancer using 67215-1-Ig (same clone as 67215-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67215-1-Ig (p130Cas / BCAR1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using p130Cas / BCAR1 Monoclonal antibody (67215-1-Ig)

    IHC staining of human breast cancer using 67215-1-Ig (same clone as 67215-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67215-1-Ig (p130Cas / BCAR1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using p130Cas / BCAR1 Monoclonal antibody (67215-1-Ig)

    IHC staining of human colon cancer using 67215-1-Ig (same clone as 67215-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67215-1-Ig (p130Cas / BCAR1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using p130Cas / BCAR1 Monoclonal antibody (67215-1-Ig)

    IHC staining of human colon cancer using 67215-1-Ig (same clone as 67215-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67215-1-Ig (p130Cas / BCAR1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using p130Cas / BCAR1 Monoclonal antibody (67215-1-Ig)

    IF Staining of human breast cancer using 67215-1-Ig (same clone as 67215-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using p130Cas / BCAR1 antibody (67215-1-Ig, Clone: 2D11C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using p130Cas / BCAR1 Monoclonal antibody (67215-1-Ig)

    IF Staining of human breast cancer using 67215-1-Ig (same clone as 67215-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using BCAR1 antibody (67215-1-Ig, Clone: 2D11C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using p130Cas / BCAR1 Monoclonal antibody (67215-1-Ig)

    FC experiment of A431 using 67215-1-Ig (same clone as 67215-1-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug p130Cas / BCAR1 Monoclonal antibody (67215-1-Ig, Clone:2D11C10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

p130Cas / BCAR1 Monoclonal antibody, PBS Only

p130Cas / BCAR1 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA
Cat No. 67215-1-PBS
Clone No.2D11C10

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Synonyms

BCAR1, 2D11C10, Breast cancer anti-estrogen resistance protein 1, CAS, CAS1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67215-1-PBS targets p130Cas / BCAR1 in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag10604

Product name: Recombinant human BCAR1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-420 aa of BC062556

Sequence: MNHLNVLAKALYDNVAESPDELSFRKGDIMTVLEQDTQGLDGWWLCSLHGRQGIVPGNRLKILVGMYDKKPAGPGSGPPATPAQPQPGLHAPAPPASQYTPMLPNTYQPQPDSVYLVPTPSKAQQGLYQVPGPSPQFQSPPAKQTSTFSKQTPHHPFPNPATDLYQVPPGPGGPAQDIYQVPPSAGMGHDIYQVPPSMDTRSWEGTKPPAKVVVPTRVGQGYVYEAAQPEQDEYDIPRHLLAPGPQDIYDVPPVRGLLPSQYGQEVYDTPPMAVKGPNGRDPLLEVYDVPPSVEKGLPPSNHHAVYDVPPSVSKDVPDGPLLREETYDVPPAFAKAKPFDPARTPLVLAAPPPDSPPAEDVYDVPPPAPDLYDVPPGLRRPGPGTLYDVPRERVLPPEVADGGVVDSGVYAVPPPAEREA

相同性解析による交差性が予測される生物種
Full Name breast cancer anti-estrogen resistance 1
Calculated molecular weight 870 aa, 93 kDa
Observed molecular weight 116 kDa
GenBank accession numberBC062556
Gene Symbol BCAR1
Gene ID (NCBI) 9564
RRIDAB_2882507
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP56945
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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