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Validation Data Gallery

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  • Western Blot (WB) analysis of fetal human brain tissue using p120 Catenin Monoclonal antibody (66208-1-Ig)

    WB analysis of fetal human brain using 66208-1-Ig (same clone as 66208-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66208-1-Ig (p120 Catenin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using p120 Catenin Monoclonal antibody (66208-1-Ig)

    WB analysis of mouse brain using 66208-1-Ig (same clone as 66208-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66208-1-Ig (p120 Catenin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using p120 Catenin Monoclonal antibody (66208-1-Ig)

    WB analysis of A549 using 66208-1-Ig (same clone as 66208-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 66208-1-Ig (p120 Catenin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using p120 Catenin Monoclonal antibody (66208-1-Ig)

    WB analysis of HeLa using 66208-1-Ig (same clone as 66208-1-PBS)

    WB result of p120 Catenin antibody (66208-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-p120 Catenin transfected HeLa cells. This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using p120 Catenin Monoclonal antibody (66208-1-Ig)

    WB analysis of HEK-293 using 66208-1-Ig (same clone as 66208-1-PBS)

    WB result of p120 Catenin antibody (66208-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-p120 Catenin transfected HEK-293 cells. This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using p120 Catenin Monoclonal antibody (66208-1-Ig)

    IHC staining of human breast cancer using 66208-1-Ig (same clone as 66208-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using p120 Catenin Monoclonal antibody (66208-1-Ig)

    IHC staining of human breast cancer using 66208-1-Ig (same clone as 66208-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using p120 Catenin Monoclonal antibody (66208-1-Ig)

    IHC staining of human breast cancer using 66208-1-Ig (same clone as 66208-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat colon tissue using p120 Catenin Monoclonal antibody (66208-1-Ig)

    IHC staining of rat colon using 66208-1-Ig (same clone as 66208-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat colon tissue using p120 Catenin Monoclonal antibody (66208-1-Ig)

    IHC staining of rat colon using 66208-1-Ig (same clone as 66208-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using p120 Catenin Monoclonal antibody (66208-1-Ig)

    IHC staining of mouse colon using 66208-1-Ig (same clone as 66208-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using p120 Catenin Monoclonal antibody (66208-1-Ig)

    IHC staining of mouse colon using 66208-1-Ig (same clone as 66208-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using p120 Catenin Monoclonal antibody (66208-1-Ig)

    IHC staining of human colon cancer using 66208-1-Ig (same clone as 66208-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using p120 Catenin Monoclonal antibody (66208-1-Ig)

    IHC staining of human colon cancer using 66208-1-Ig (same clone as 66208-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using p120 Catenin Monoclonal antibody (66208-1-Ig)

    IF Staining of human breast cancer using 66208-1-Ig (same clone as 66208-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using p120 Catenin Monoclonal antibody (66208-1-Ig)

    IF Staining of human breast cancer using 66208-1-Ig (same clone as 66208-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse breast cancer using p120 Catenin Monoclonal antibody (66208-1-Ig)

    IF Staining of mouse breast cancer using 66208-1-Ig (same clone as 66208-1-PBS)

    Immunofluorescent analysis of un-fixed frozen OCT-embedded mouse breast cancer using p120 Catenin antibody (66208-1-Ig, Clone: 2F7H8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse breast cancer using p120 Catenin Monoclonal antibody (66208-1-Ig)

    IF Staining of mouse breast cancer using 66208-1-Ig (same clone as 66208-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse breast cancer using p120 Catenin antibody (66208-1-Ig, Clone: 2F7H8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using p120 Catenin Monoclonal antibody (66208-1-Ig)

    IF Staining of A431 using 66208-1-Ig (same clone as 66208-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using p120 Catenin antibody (66208-1-Ig, Clone: 2F7H8 ) at dilution of 1:800 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using p120 Catenin Monoclonal antibody (66208-1-Ig)

    IF Staining of HeLa using 66208-1-Ig (same clone as 66208-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66208-1-Ig(p120 Catenin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

p120 Catenin Monoclonal antibody, PBS Only

p120 Catenin Monoclonal Antibody for WB, IHC, IF/ICC, IF-Fro, Indirect ELISA
Cat No. 66208-1-PBS
Clone No.2F7H8

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-Fro, Indirect ELISA

Synonyms

CTNND1, Cadherin-associated Src substrate, CAS, Catenin delta 1, Catenin delta-1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66208-1-PBS targets p120 Catenin in WB, IHC, IF/ICC, IF-Fro, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag2824

Product name: Recombinant human CTNND1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 546-830 aa of BC010501

Sequence: GYELLFQPEVVRIYISLLKESKTPAILEASAGAIQNLCAGRWTYGRYIRSALRQEKALSAIADLLTNEHERVVKAASGALRNLAVDARNKELIGKHAIPNLVKNLPGGQQNSSWNFSEDTVISILNTINEVIAENLEAAKKLRETQGIEKLVLINKSGNRSEKEVRAAALVLQTIWGYKELRKPLEKEGWKKSDFQVNLNNASRSQSSHSYDDSTLPLIDRNQKSDKKPDREEIQMSNMGSNTKSLDNNYSTPNERGDHNKTLDRSGDLGDMEPLKGTTPLMQKI

相同性解析による交差性が予測される生物種
Full Name catenin (cadherin-associated protein), delta 1
Calculated molecular weight 948 aa, 105 kDa
Observed molecular weight 90-120 kDa
GenBank accession numberBC010501
Gene Symbol p120 Catenin
Gene ID (NCBI) 1500
RRIDAB_2881599
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO60716
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Catenins were discovered as proteins that are linked to the cytoplasmic domain of transmembrane cadherins (PMID: 9653641). p120 catenin, also called p120 ctn or catenin delta-1, regulates cell-cell adhesion through its interaction with the cytoplasmic tail of classical and type II cadherins. p120 catenin is a tyrosine kinase substrate implicated in cell transformation by SRC, as well as in ligand-induced receptor signaling through the EGF receptor, the PDGF receptor, and the CSF1 receptor. Different expression patterns of p120 catenin in lobular and ductal carcinomas of breast have been reported: membrane stain for ductal carcinoma and cytoplasmic stain for lobular carcinoma (PMID: 24966968). Different isoforms of p120 catenin are variably expressed in different tissues as a result of alternative splicing and the use of multiple translation initiation codons (PMID: 19150613).

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