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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using p115, USO1 Monoclonal antibody (68100-1-Ig)

    WB analysis using 68100-1-Ig (same clone as 68100-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68100-1-Ig (p115, USO1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 68100-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using p115, USO1 Monoclonal antibody (68100-1-Ig)

    WB analysis of HeLa using 68100-1-Ig (same clone as 68100-1-PBS)

    WB result of p115, USO1 antibody (68100-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-p115, USO1 transfected HeLa cells. This data was developed using the same antibody clone with 68100-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using p115, USO1 Monoclonal antibody (68100-1-Ig)

    IHC staining of human liver cancer using 68100-1-Ig (same clone as 68100-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68100-1-Ig (p115, USO1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68100-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using p115, USO1 Monoclonal antibody (68100-1-Ig)

    IHC staining of human liver cancer using 68100-1-Ig (same clone as 68100-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68100-1-Ig (p115, USO1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68100-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using p115, USO1 Monoclonal antibody (68100-1-Ig)

    IF Staining of HeLa using 68100-1-Ig (same clone as 68100-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using p115, USO1 antibody (68100-1-Ig, Clone: 3B7D8 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68100-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using p115, USO1 Monoclonal antibody (68100-1-Ig)

    IF Staining of HeLa using 68100-1-Ig (same clone as 68100-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using p115, USO1 antibody (68100-1-Ig, Clone: 3B7D8 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68100-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

p115, USO1 Monoclonal antibody, PBS Only

p115, USO1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 68100-1-PBS
Clone No.3B7D8

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

USO1, 3B7D8, General vesicular transport factor p115, Protein USO1 homolog, TAP

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68100-1-PBS targets p115, USO1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag5543

Product name: Recombinant human p115, USO1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 660-961 aa of BC032654

Sequence: EQDLQLEELRQQVSTLKCQNEQLQTAVTQQVSQIQQHKDQYNLLKIQLGKDNQHQGSYSEGAQMNGIQPEEIGRLREEIEELKRNQELLQSQLTEKDSMIENMKSSQTSGTNEQSSAIVSARDSEQVAELKQELATLKSQLNSQSVEITKLQTEKQELLQKTEAFAKSVEVQGETETIIATKTTDVEGRLSALLQETKELKNEIKALSEERTAIKEQLDSSNSTIAILQTEKDKLELEITDSKKEQDDLLVLLADQDQKILSLKNKLKDLGHPVEEEDELESGDQEDEDDESEDPGKDLDHI

相同性解析による交差性が予測される生物種
Full Name USO1 homolog, vesicle docking protein (yeast)
Calculated molecular weight 962 aa, 108 kDa
Observed molecular weight108 kDa
GenBank accession numberBC032654
Gene Symbol USO1
Gene ID (NCBI) 8615
RRIDAB_2918835
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO60763
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

p115, also known as USO1, TAP (transcytosis-associated protein) or VDP (vesicle docking protein) is a general vesicular transport factor and plays an important role at different steps of vesicular transport. It is a 962-residue peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase (PMID: 9478999). p115 forms stable homodimers (PMID: 19247479). Rab1 recruits p115 to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where p115 interacts directly with a select set of SNARE proteins (PMID: 10903204). p115 is required for intra-Golgi transport, and also functions in endoplasmic reticulum to Golgi trafficking, Golgi biogenesis and exocytotic transport (PMID: 19247479).

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