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ChromoTek mNeonGreen Chromobody® plasmid (mCherry)

DNA plasmid encoding for anti-mNeonGreen VHH (Nanobody) fused to fluorescent protein mCherry.

Reactivity

mNeonGreen

Applications

IF, Live Cell Imaging

Vector Type

Mammalian expression vector

Reporter/Conjugate

mCherry

Cat no : mcr

Synonyms

Chromobody, mNeonGreen Chromobody, mNeonGreen Live Cell, Live-Cell Imaging, Live Cell Imaging, Live Cell, Live-Cell

Validation Data Gallery

  • Fluorescence microscopy of HeLa cells, transiently expressing Vimentin-mNeonGreen (green) and the mNeonGreen-Chromobody-(mCherry) (red). Nuclei are stained with DAPI (cyan). Green and red fluorescent signals co-localize in co-transfected cells, indicating enrichment of the Chromobody at the mNeonGreen-positive structures. Confocal images were were acquired with a 63x oil objective and post-processed.

    Fluorescence microscopy of HeLa cells, transiently expressing Vimentin-mNeonGreen (green) and the mNeonGreen-Chromobody-(mCherry) (red). Nuclei are stained with DAPI (cyan). Green and red fluorescent signals co-localize in co-transfected cells, indicating enrichment of the Chromobody at the mNeonGreen-positive structures. Confocal images were were acquired with a 63x oil objective and post-processed.

Product Information

DNA plasmid encoding for anti-mNeonGreen VHH (Nanobody) fused to fluorescent protein mCherry.

ApplicationsIF, Live Cell Imaging
ReactivitymNeonGreen
Reporter/ConjugatemCherry
Vector typeMammalian expression vector
PromoterCMV
Codon usageMammalian
SelectionKan/Neo
Clone#CTK0234
ClassChromobody
Affinity17 kDa
Molecular Weight41.3 kDa
TypeChromobody
HostAlpaca
ImmunogenmNeonGreen
Excitation/Emission Maxima Wavelengths587 nm / 610 nm
FormLiquid
Storage bufferH2O
Shippingambient temperature
Storage condition-20°C
TransfectionTransfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.