Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 80517-1-RR (same clone as 80517-1-PBS)
Untreated and LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 80517-1-RR (iNOS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.
WB analysis of human PBMCs using 80517-1-RR (same clone as 80517-1-PBS)
LPS treated human PBMCs were subjected to SDS PAGE followed by western blot with 80517-1-RR (iNOS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 80517-1-RR (same clone as 80517-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 80517-1-RR (same clone as 80517-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 80517-1-RR (same clone as 80517-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 80517-1-RR (same clone as 80517-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 80517-1-RR (same clone as 80517-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 80517-1-RR (same clone as 80517-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 80517-1-RR (same clone as 80517-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 80517-1-RR (same clone as 80517-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 80517-1-RR (same clone as 80517-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 80517-1-RR (same clone as 80517-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.
IHC staining of human lung using 80517-1-RR (same clone as 80517-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.
IHC staining of human lung using 80517-1-RR (same clone as 80517-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.
IF Staining of RAW 264.7 using 80517-1-RR (same clone as 80517-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using iNOS antibody (80517-1-RR, Clone: 6O22 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.

iNOS Recombinant monoclonal antibody, PBS Only

iNOS Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 80517-1-PBS

Clone No.6O22

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

NOS2, 6O22, EC:1.14.13.39, Hepatocyte NOS, HEP-NOS

Formulation: PBS Only

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80517-1-PBS targets iNOS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	nitric oxide synthase 2, inducible
Calculated molecular weight	131 kDa
Observed molecular weight	110-130 kDa
GenBank accession number	NM_000625
Gene Symbol	iNOS
Gene ID (NCBI)	4843
RRID	AB_2918898
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P35228
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

NOS2, also named as iNOS and NOS2A, produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. NO is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission, antimicrobial and antitumoral activities. NOS2 is a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. iNOS has a very short half-life due to rapid degradation by calpain. iNOS monomer is a direct substrate of calpain I and can be cleaved by calpain I at the canonical CaM-binding site(503-532aa) of iNOS, and then a ~70kD band can be detected by western (PMID:11786228). This antibody is specific to NOS2.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 80517-1-RR (same clone as 80517-1-PBS)

WB analysis of human PBMCs using 80517-1-RR (same clone as 80517-1-PBS)

IHC staining of mouse brain using 80517-1-RR (same clone as 80517-1-PBS)

IHC staining of mouse brain using 80517-1-RR (same clone as 80517-1-PBS)

IHC staining of human colon using 80517-1-RR (same clone as 80517-1-PBS)

IHC staining of rat brain using 80517-1-RR (same clone as 80517-1-PBS)

IHC staining of rat brain using 80517-1-RR (same clone as 80517-1-PBS)

IHC staining of human colon using 80517-1-RR (same clone as 80517-1-PBS)

IHC staining of human liver using 80517-1-RR (same clone as 80517-1-PBS)

IHC staining of human liver using 80517-1-RR (same clone as 80517-1-PBS)

IHC staining of human colon using 80517-1-RR (same clone as 80517-1-PBS)

IHC staining of human colon using 80517-1-RR (same clone as 80517-1-PBS)

IHC staining of human lung using 80517-1-RR (same clone as 80517-1-PBS)

IHC staining of human lung using 80517-1-RR (same clone as 80517-1-PBS)

IF Staining of RAW 264.7 using 80517-1-RR (same clone as 80517-1-PBS)

iNOS Recombinant monoclonal antibody, PBS Only

iNOS Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Product Information

Background Information

Able™

iNOS Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Able^™