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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using human IgM Polyclonal antibody (11016-1-AP)

    WB analysis of human plasma using 11016-1-AP (same clone as 11016-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 11016-1-AP (IGHM Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11016-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using human IgM Polyclonal antibody (11016-1-AP)

    IHC staining of human tonsillitis using 11016-1-AP (same clone as 11016-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11016-1-AP (IGHM antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11016-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using human IgM Polyclonal antibody (11016-1-AP)

    IHC staining of human tonsillitis using 11016-1-AP (same clone as 11016-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11016-1-AP (IGHM antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11016-1-PBS in a different storage buffer formulation.

human IgM Polyclonal antibody, PBS Only

human IgM Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 11016-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

IgM, IGHM, VH, MU

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11016-1-PBS targets human IgM in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1459

Product name: Recombinant human IGHM protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-351 aa of BC009851

Sequence: FGNPRKSKLICQATGFSPRQIQVSWLREGKQVGSGVTTDQVQAEAKESGPTTYKVTSTLTIKESDWLSQSMFTCRVDHRGLTFQQNASSMCVPDQDTAIRVFAIPPSFASIFLTKSTKLTCLVTDLTTYDSVTISWTRQNGEAVKTHTNISESHPNATFSAVGEASICEDDWNSGERFTCTVTHTDLPSPLKQTISRPKGVALHRPDVYLLPPAREQLNLRESATITCLVTGFSPADVFVQWMQRGQPLSPEKYVTSAPMPEPQAPGRYFAHSILTVSEEEWNTGETYTCVVAHEALPNRVTERTVDKSTEGEVSADEEGFENLWATASTFIVLFLLSLFYSTTVTLFKVK

相同性解析による交差性が予測される生物種
Full Name immunoglobulin heavy constant mu
Calculated molecular weight 69 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC009851
Gene Symbol IGHM
Gene ID (NCBI) 3507
RRIDAB_593462
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

This antibody detects the heavy chain of human IgM (~75 kDa).

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