Recombinant Human ICOS/CD278 protein (hFc Tag, Myc Tag, His Tag)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
hFc Tag, Myc Tag, His Tag
Activity
EC50: 6-24 ng/mL
Cat no : Eg0043
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Immobilized Human ICOSLG (Myc tag, His tag) at 0.5 μg/mL (100 μL/well) can bind Human ICOS (hFc tag, Myc tag, His tag) with a linear range of 6-24 ng/mL. |
| Expression | HEK293-derived Human ICOS protein Glu21-Lys140 (Accession# Q9Y6W8) with a human IgG1 Fc tag, a Myc tag,a His tag at the C-terminus. |
| GeneID | 29851 |
| Accession | Q9Y6W8 |
| PredictedSize | 42.1 kDa |
| SDS-PAGE | 50-60 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
Inducible T-cell costimulator (ICOS) is an activation-dependent, T cell-specific cell surface molecule related to CD28 and CTLA-4. ICOS enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. ICOS has to be de novo induced on the T-cell surface, does not upregulate the production of interleukin-2, but superinduces the synthesis of interleukin-10, a B-cell-differentiation factor. ICOS activated T-cells, Highly expressed on tonsillar T-cells, are closely associated with B-cells in the apical light zone of germinal centers, the site of terminal B-cell maturation. It is expressed at lower levels in thymus, lung, lymph node, and peripheral blood leukocytes. It is also expressed in the medulla of the fetal and newborn thymus.
References:
1. Beier KC, et al. (2000). Eur J Immunol. 30(12):3707-17. 2. Hutloff A, et al. (1999). Nature. 397(6716):263-266. 3. Tezuka K, et al. (2000). Biochem Biophys Res Commun. 276(1):335-345.