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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PCBP1 Polyclonal antibody (14523-1-AP)

    WB analysis using 14523-1-AP (same clone as 14523-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14523-1-AP (hnRNP-E1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14523-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using PCBP1 Polyclonal antibody (14523-1-AP)

    WB analysis of HepG2 using 14523-1-AP (same clone as 14523-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14523-1-AP (hnRNP-E1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14523-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PCBP1 Polyclonal antibody (14523-1-AP)

    WB analysis of HeLa using 14523-1-AP (same clone as 14523-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 14523-1-AP (hnRNP-E1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14523-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using PCBP1 Polyclonal antibody (14523-1-AP)

    WB analysis of K-562 using 14523-1-AP (same clone as 14523-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 14523-1-AP (hnRNP-E1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14523-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using PCBP1 Polyclonal antibody (14523-1-AP)

    WB analysis of MCF-7 using 14523-1-AP (same clone as 14523-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 14523-1-AP (hnRNP-E1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14523-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PCBP1 Polyclonal antibody (14523-1-AP)

    IHC staining of human lung cancer using 14523-1-AP (same clone as 14523-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14523-1-AP (hnRNP-E1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14523-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PCBP1 Polyclonal antibody (14523-1-AP)

    IHC staining of human lung cancer using 14523-1-AP (same clone as 14523-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14523-1-AP (hnRNP-E1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14523-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PCBP1 Polyclonal antibody (14523-1-AP)

    IF Staining of HepG2 using 14523-1-AP (same clone as 14523-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using hnRNP-E1 antibody (14523-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14523-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using PCBP1 Polyclonal antibody (14523-1-AP)

    IF Staining of NIH/3T3 using 14523-1-AP (same clone as 14523-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using hnRNP-E1 antibody (14523-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14523-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using PCBP1 Polyclonal antibody (14523-1-AP)

    IF Staining of A431 using 14523-1-AP (same clone as 14523-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using hnRNP-E1 antibody (14523-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14523-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PCBP1 Polyclonal antibody (14523-1-AP)

    IF Staining of HeLa using 14523-1-AP (same clone as 14523-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using hnRNP-E1 antibody (14523-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 14523-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PCBP1 Polyclonal antibody, PBS Only

PCBP1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 14523-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

hnRNP E1, hnRNP E1/PCBP1, hnRNP-E1, Alpha CP1, Alpha-CP1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14523-1-PBS targets PCBP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6007

Product name: Recombinant human hnRNP-E1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-356 aa of BC039742

Sequence: MDAGVTESGLNVTLTIRLLMHGKEVGSIIGKKGESVKRIREESGARINISEGNCPERIITLTGPTNAIFKAFAMIIDKLEEDINSSMTNSTAASRPPVTLRLVVPATQCGSLIGKGGCKIKEIRESTGAQVQVAGDMLPNSTERAITIAGVPQSVTECVKQICLVMLETLSQSPQGRVMTIPYQPMPASSPVICAGGQDRCSDAAGYPHATHDLEGPPLDAYSIQGQHTISPLDLAKLNQVARQQSHFAMMHGGTGFAGIDSSSPEVKGYWASLDASTQTTHELTIPNNLIGCIIGRQGANINEIRQMSGAQIKIANPVEGSSGRQVTITGSAASISLAQYLINARLSSEKGMGCS

相同性解析による交差性が予測される生物種
Full Name poly(rC) binding protein 1
Calculated molecular weight 37 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC039742
Gene Symbol PCBP1
Gene ID (NCBI) 5093
RRIDAB_2160619
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15365
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

hnRNP-E1 (heterogeneous nuclear ribonucleoprotein E1) is also named as PCBP1 and Alpha-CP1. Poly(rC)-binding protein 1 (PCBP1 also called hnRNP E1) belongs to a hnRNP family that is composed of hnRNP K/J and the alpha-complex proteins (PCBP1-4α or CP1-4). Proteins in this family contain three hnRNP K homology (KH) domains for RNA-binding. The human PCBP1 gene encodes the hnRNP E1 protein, and was initially defined as clone sub2.3, with poly(C)-binding activity and observed similarity to hnRNP E2 (PMID: 8152927). hnRNP-E1 is an RNA binding protein that regulates diverse processes including translation and splicing (PMID:29396052). hnRNP E1 regulates splicing of EMT-associated genes including CD44 and PNUTS (PMID: 28825698). This antibody recognizes hnRNP E1/E2.

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