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Validation Data Gallery

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  • Western Blot (WB) analysis of SMMC-7721 cells using golgin 97 Polyclonal antibody (12640-1-AP)

    WB analysis of SMMC-7721 using 12640-1-AP (same clone as 12640-1-PBS)

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 12640-1-AP (golgin 97 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12640-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using golgin 97 Polyclonal antibody (12640-1-AP)

    WB analysis using 12640-1-AP (same clone as 12640-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12640-1-AP (golgin 97 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12640-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using golgin 97 Polyclonal antibody (12640-1-AP)

    WB analysis using 12640-1-AP (same clone as 12640-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12640-1-AP (golgin 97 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12640-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using golgin 97 Polyclonal antibody (12640-1-AP)

    IF Staining of HeLa using 12640-1-AP (same clone as 12640-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using golgin 97 antibody (12640-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12640-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using golgin 97 Polyclonal antibody (12640-1-AP)

    IF Staining of HeLa using 12640-1-AP (same clone as 12640-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using golgin 97 antibody (12640-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 12640-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using golgin 97 Polyclonal antibody (12640-1-AP)

    FC experiment of HeLa using 12640-1-AP (same clone as 12640-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human golgin 97 (12640-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 12640-1-PBS in a different storage buffer formulation.

golgin 97 Polyclonal antibody, PBS Only

golgin 97 Polyclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 12640-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12640-1-PBS targets golgin 97 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3335

Product name: Recombinant human GOLGA1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 418-767 aa of BC032853

Sequence: QIVALERMRAADQTTAEQGMRQLEQENAALKECRNEYERSLQNHQFELKKLKEEWSQREIVSVAMAQALEEVRKQREEFQQQAANLTAIIDEKEQNLREKTEVLLQKEQEILQLERGHNSALLQIHQLQAELEALRTLKAEEAAVVAEQEDLLRLRGPLQAEALSVNESHVTSRAMQDPVFQLPTAGRTPNGEVGAMDLTQLQKEKQDLEQQLLEKNKTIKQMQQRMLELRKTLQKELKIRPDNELFEVREKPGPEMANMAPSVTNNTDLTDAREINFEYLKHVVLKFMSCRESEAFHLIKAVSVLLNFSQEEENMLKETLEYKMSWFGSKPAPKGSIRPSISNPRIPWS

相同性解析による交差性が予測される生物種
Full Name golgi autoantigen, golgin subfamily a, 1
Calculated molecular weight 767 aa, 88 kDa
Observed molecular weight 97 kDa
GenBank accession numberBC032853
Gene Symbol golgin 97
Gene ID (NCBI) 2800
RRIDAB_2115315
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92805
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Golgin subfamily A member 1 is a protein that in humans is encoded by the GOLGA1 gene. GOLGA1 has been shown to interact with ARL1. GOLGA1 is associated with Sjogren's syndrome. The MW of this protein is 97 kDa, and Catalog#12640-1-AP specially recognises the 97 kDa protein. IF result shows strong Gogli staining.

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