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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using Gephyrin/GPHN Polyclonal antibody (12681-1-AP)

    WB analysis of HEK-293 using 12681-1-AP (same clone as 12681-1-PBS)

    WB result of gephyrin antibody (12681-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-gephyrin transfected HEK-293 cells. This data was developed using the same antibody clone with 12681-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Gephyrin/GPHN Polyclonal antibody (12681-1-AP)

    WB analysis using 12681-1-AP (same clone as 12681-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12681-1-AP (gephyrin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12681-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Gephyrin/GPHN Polyclonal antibody (12681-1-AP)

    WB analysis using 12681-1-AP (same clone as 12681-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12681-1-AP (gephyrin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12681-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using Gephyrin/GPHN Polyclonal antibody (12681-1-AP)

    IP experiment of HEK-293 using 12681-1-AP (same clone as 12681-1-PBS)

    IP result of anti-gephyrin (IP:12681-1-AP, 4ug; Detection:12681-1-AP 1:700) with HEK-293 cells lysate 3200ug. This data was developed using the same antibody clone with 12681-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using Gephyrin/GPHN Polyclonal antibody (12681-1-AP)

    IHC staining of rat brain using 12681-1-AP (same clone as 12681-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12681-1-AP (gephyrin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12681-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using Gephyrin/GPHN Polyclonal antibody (12681-1-AP)

    IHC staining of rat brain using 12681-1-AP (same clone as 12681-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12681-1-AP (gephyrin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12681-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

Gephyrin/GPHN Polyclonal antibody, PBS Only

Gephyrin/GPHN Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 12681-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

gephyrin, GPHN, Domain E, Domain G, GEPH

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12681-1-PBS targets Gephyrin/GPHN in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3370

Product name: Recombinant human GPHN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-315 aa of BC030016

Sequence: MATEGMILTNHDHQIRVGVLTVSDSCFRNLAEDRSGINLKDLVQDPSLLGGTISAYKIVPDEIEEIKETLIDWCDEKELNLILTTGGTGFAPRDVTPEATKEVIEREAPGMALAMLMGSLNVTPLGMLSRPVCGIRGKTLIINLPGSKKGSQECFQFILPALPHAIDLLRDAIVKVKEVHDELEDLPSPPPPLSPPPTTSPHKQTEDKGVQCEEEEEEKKDSGVASTEDSSSSHITAAAIAAKKHPFYTSPAVVMAHGEQPIPGLINYSHHSTDERIPDSIISRGVQVLPRDTASLSTTPSESPRAQATSRLSTA

相同性解析による交差性が予測される生物種
Full Name gephyrin
Calculated molecular weight 769 aa, 83 kDa
Observed molecular weight 93 kDa
GenBank accession numberBC030016
Gene Symbol GPHN
Gene ID (NCBI) 10243
RRIDAB_2247770
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NQX3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Gephyrin (GPHN) is an organizational protein that clusters and localizes the inhibitory glycine receptor (GlyR) and GABAA receptors to the microtubular matrix of the neuronal postsynaptic membrane. Mice deficient in gephyrin develop a hereditary molybdenum cofactor deficiency and a neurological phenotype that mimics startle disease (hyperekplexia). In non-neuronal tissues, the encoded protein is also required for molybdenum cofactor biosynthesis. Two isoforms produced by alternative splicing have been described. The observed MW of Gephyrin is 93 kDa, larger than the predicated of 83 kDa, which may be due to the modifications on various phosphorylation sites.

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