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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using fetuin-B Polyclonal antibody (18052-1-AP)

    WB analysis of human plasma using 18052-1-AP

    human blood were subjected to SDS PAGE followed by western blot with 18052-1-AP (fetuin-B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human plasma using fetuin-B Polyclonal antibody (18052-1-AP)

    WB analysis of human plasma using 18052-1-AP

    human blood were subjected to SDS PAGE followed by western blot with 18052-1-AP (fetuin-B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of human plasma using fetuin-B Polyclonal antibody (18052-1-AP)

    IP experiment of human plasma using 18052-1-AP

    IP result of anti-fetuin-B (IP:18052-1-AP, 4ug; Detection:18052-1-AP 1:2000) with human plasma lysate 4000 ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using fetuin-B Polyclonal antibody (18052-1-AP)

    IHC staining of human liver cancer using 18052-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18052-1-AP (fetuin-B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using fetuin-B Polyclonal antibody (18052-1-AP)

    IHC staining of human liver cancer using 18052-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18052-1-AP (fetuin-B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using fetuin-B Polyclonal antibody (18052-1-AP)

    IHC staining of mouse liver using 18052-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver using 18052-1-AP (fetuin-B antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using fetuin-B Polyclonal antibody (18052-1-AP)

    IHC staining of mouse liver using 18052-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver using 18052-1-AP (fetuin-B antibody) at dilution of 1:50 (under 40x lens).

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fetuin-B Polyclonal antibody

fetuin-B Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 18052-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

FETUB, 16G2, Gugu, Fetuin-like protein IRL685, FetuinB

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman blood tissue, human plasma
Positive IP detected inhuman plasma tissue
Positive IHC detected inhuman liver cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18052-1-AP targets fetuin-B in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen fetuin-B fusion protein Ag12562 相同性解析による交差性が予測される生物種
Full Name fetuin B
Calculated molecular weight 382 aa, 42 kDa
Observed molecular weight 50-60 kDa
GenBank accession numberBC074734
Gene Symbol Fetuin B
Gene ID (NCBI) 26998
RRIDAB_2103181
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UGM5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fetuins A and B are both members of the cystatin superfamily. They are mainly expressed in the liver (hepatokines), as well as other tissues such as the kidneys, tongue, and placenta. Fetuin-B has been identified as a novel adipokine/hepatokine that significantly increases hepatic steatosis and mediates impaired insulin action and glucose intolerance. (PMID: 36915725, PMID: 35702137)

Protocols

Product Specific Protocols
WB protocol for fetuin-B antibody 18052-1-APDownload protocol
IHC protocol for fetuin-B antibody 18052-1-APDownload protocol
IP protocol for fetuin-B antibody 18052-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols