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Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using Cleaved GSDME (Asp270), C-terminal Recombinant mon (88249-1-RR)

    WB analysis of A431 using 88249-1-RR

    Doxorubicin hydrochloride treated A431 cells were subjected to SDS PAGE followed by western blot with 88249-1-RR (Cleaved GSDME (Asp270), C-terminal antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using Cleaved GSDME (Asp270), C-terminal Recombinant mon (88249-1-RR)

    WB analysis of SH-SY5Y using 88249-1-RR

    etoposide treated SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 88249-1-RR (Cleaved GSDME (Asp270), C-terminal antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using Cleaved GSDME (Asp270), C-terminal Recombinant mon (88249-1-RR)

    FC experiment of SH-SY5Y using 88249-1-RR

    1X10^6 SH-SY5Y cells untreated (dashed lines) or treated with etoposide which intracellularly stained with 0.25 ug cleaved GSDME (Asp270), C-terminal Recombinant monoclonal antibody (88249-1-RR, Clone:253373H3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

Cleaved GSDME (Asp270), C-terminal Recombinant monoclonal antibody

Cleaved GSDME (Asp270), C-terminal Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 88249-1-RR
Clone No.253373H3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

Cleaved Gasdermin E, cleaved GSDME, cleaved GSDME (Asp270), DFNA5, DFNA5/GSDME

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inDoxorubicin hydrochloride treated A431 cells, etoposide treated SH-SY5Y cells
Positive FC (Intra) detected inetoposide treated SH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

88249-1-RR targets Cleaved GSDME (Asp270), C-terminal in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name deafness, autosomal dominant 5
Calculated molecular weight 496 aa, 55 kDa
Observed molecular weight25 kDa
GenBank accession numberBC019689
Gene Symbol DFNA5
Gene ID (NCBI) 1687
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO60443
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GSDME, also known as DFNA5 (deafness, autosomal dominant 5), is a member of the gasdermin family of pore-forming proteins. In its full-length form, GSDME is autoinhibited. Upon apoptotic stimulation, active caspase-3 specifically cleaves GSDME at a conserved aspartate residue, Asp270 (D270). This cleavage of Gasdermin E separates the N‑terminal pore‑forming domain from the C‑terminal repressor domain. The released N‑terminal fragment then inserts into the plasma membrane, forms large pores, and induces a lytic, inflammatory form of cell death known as pyroptosis. Therefore, Asp270 cleavage is a critical molecular switch that converts caspase‑3‑mediated apoptosis into pyroptosis, linking GSDME/DFNA5 to cancer therapy, chemotherapy‑induced toxicity, and hearing loss pathology.

Protocols

Product Specific Protocols
FC protocol for Cleaved GSDME (Asp270), C-terminal antibody 88249-1-RRDownload protocol
WB protocol for Cleaved GSDME (Asp270), C-terminal antibody 88249-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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