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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using calreticulin Recombinant antibody (80017-1-RR)

    WB analysis using 80017-1-RR (same clone as 80017-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80017-1-RR (calreticulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80017-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using calreticulin Recombinant antibody (80017-1-RR)

    WB analysis of NIH/3T3 using 80017-1-RR (same clone as 80017-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80017-1-RR (calreticulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80017-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using calreticulin Recombinant antibody (80017-1-RR)

    WB analysis of HSC-T6 using 80017-1-RR (same clone as 80017-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 80017-1-RR (calreticulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80017-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using calreticulin Recombinant antibody (80017-1-RR)

    IHC staining of human thyroid cancer using 80017-1-RR (same clone as 80017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 80017-1-RR (calreticulin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80017-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using calreticulin Recombinant antibody (80017-1-RR)

    IHC staining of human thyroid cancer using 80017-1-RR (same clone as 80017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 80017-1-RR (calreticulin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80017-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using calreticulin Recombinant antibody (80017-1-RR)

    IHC staining of human breast cancer using 80017-1-RR (same clone as 80017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80017-1-RR (calreticulin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80017-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using calreticulin Recombinant antibody (80017-1-RR)

    IHC staining of human breast cancer using 80017-1-RR (same clone as 80017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80017-1-RR (calreticulin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80017-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80017-1-RR

    Affinity and Kinetic Characterization of 80017-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80017-1-RR against Human calreticulin were performed. The affinity constant is 6.07 nM.

calreticulin Recombinant monoclonal antibody, PBS Only

calreticulin Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 80017-1-PBS
Clone No.2B17

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

CALR, Calregulin, 2B17

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80017-1-PBS targets calreticulin in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag26064

Product name: Recombinant human calreticulin protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 248-417 aa of BC002500

Sequence: KKPEDWDEEMDGEWEPPVIQNPEYKGEWKPRQIDNPDYKGTWIHPEIDNPEYSPDPSIYAYDNFGVLGLDLWQVKSGTIFDNFLITNDEAYAEEFGNETWGVTKAAEKQMKDKQDEEQRLKEEEEDKKRKEEEEAEDKEDDEDKDEDEEDEEDKEEDEEEDVPGQAKDEL

相同性解析による交差性が予測される生物種
Full Name calreticulin
Calculated molecular weight 60 kDa
Observed molecular weight50-55 kDa
GenBank accession numberBC002500
Gene Symbol Calreticulin
Gene ID (NCBI) 811
RRIDAB_2882941
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP27797
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CALR,also named as grp60, ERp60, HACBP, CRP55, CRTC and Calregulin, belongs to the calreticulin family. It is a molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. CALR is a ER marker. It interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. CALR interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. The MW of CALR migrates aberrantly at 55 kDa by SDS-PAGE. Some study provided that it's a new possibility for CRT-mediated tumor immune prevention and treatment.

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