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Validation Data Gallery

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  • Sandwich ELISA sample test of MP02279-2

    Sandwich ELISA sample test of MP02279-2

    The mean TGFBI/BIGH3 concentration was determined to be 136.1 ng/mL in HUVEC cell extract based on a 2.2 mg/mL extract load.

  • Sandwich ELISA sample test of MP02279-2

    Sandwich ELISA sample test of MP02279-2

    HUVEC cells were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernatant was removed, assayed for human TGFBI/BIGH3, and measured 7.9 ng/mL.

  • Sandwich ELISA sample test of MP02279-2

    Sandwich ELISA sample test of MP02279-2

    Plasma of six individual healthy human donors was measured. The TGFBI/BIGH3 concentration of detected samples was determined to be 1,016.5 ng/mL with a range of 640.9-1,282.5 ng/mL.

  • Sandwich ELISA standard curve of MP02279-2

    Sandwich ELISA standard curve of MP02279-2

    Sandwich ELISA standard curve of MP02279-2, Human TGFBI/BIGH3 Recombinant Matched Antibody Pair - PBS only. 80805-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1233. 80805-2-PBS was HRP conjugated as the detection antibody. Range: 97.7-6250 pg/mL.

  • Cytometric bead array sample test of MP02279-1

    Cytometric bead array sample test of MP02279-1

    The mean TGFBI/BIGH3 concentration was determined to be 3586.8 ng/mL in human serum.

  • Cytometric bead array standard curve of MP02279-1

    Cytometric bead array standard curve of MP02279-1

    Cytometric bead array standard curve of MP02279-1, TGFBI/BIGH3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 80805-3-PBS. Detection antibody: 80805-2-PBS. Standard: Eg1233. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 80805-2-PBS

    Affinity and Kinetic Characterization of 80805-2-PBS

    Biolayer interferometry (BLl) kinetic assays of 80805-2-PBS against Human beta IG-H3/TGFBI were performed. The affinity constant is 1.41 nM.

beta IG-H3/TGFBI Recombinant monoclonal antibody, PBS Only (Detector)

beta IG-H3/TGFBI Uni-rAb® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA
Cat No. 80805-2-PBS
Clone No.243108E6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA

Synonyms

TGFBI, TGFBI / BIGH3, Beta ig h3, Beta ig-h3, BIGH3

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80805-2-PBS targets beta IG-H3/TGFBI as part of a matched antibody pair:

MP02279-1: 80805-3-PBS capture and 80805-2-PBS detection (validated in Cytometric bead array)

MP02279-2: 80805-5-PBS capture and 80805-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg1233

Product name: recombinant human beta IG-H3/TGFBI protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 24-683 aa of NM_000358.3

Sequence: GPAKSPYQLVLQHSRLRGRQHGPNVCAVQKVIGTNRKYFTNCKQWYQRKICGKSTVISYECCPGYEKVPGEKGCPAALPLSNLYETLGVVGSTTTQLYTDRTEKLRPEMEGPGSFTIFAPSNEAWASLPAEVLDSLVSNVNIELLNALRYHMVGRRVLTDELKHGMTLTSMYQNSNIQIHHYPNGIVTVNCARLLKADHHATNGVVHLIDKVISTITNNIQQIIEIEDTFETLRAAVAASGLNTMLEGNGQYTLLAPTNEAFEKIPSETLNRILGDPEALRDLLNNHILKSAMCAEAIVAGLSVETLEGTTLEVGCSGDMLTINGKAIISNKDILATNGVIHYIDELLIPDSAKTLFELAAESDVSTAIDLFRQAGLGNHLSGSERLTLLAPLNSVFKDGTPPIDAHTRNLLRNHIIKDQLASKYLYHGQTLETLGGKKLRVFVYRNSLCIENSCIAAHDKRGRYGTLFTMDRVLTPPMGTVMDVLKGDNRFSMLVAAIQSAGLTETLNREGVYTVFAPTNEAFRALPPRERSRLLGDAKELANILKYHIGDEILVSGGIGALVRLKSLQGDKLEVSLKNNVVSVNKEPVAEPDIMATNGVVHVITNVLQPPANRPQERGDELADSALEIFKQASAFSRASQRSVRLAPVYQKLLERMKH

相同性解析による交差性が予測される生物種
Full Name transforming growth factor, beta-induced, 68kDa
Calculated molecular weight75kDa
GenBank accession numberNM_000358.3
Gene Symbol TGFBI
Gene ID (NCBI) 7045
ENSEMBL Gene IDENSG00000120708
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ15582
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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