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Beta Catenin Polyclonal antibody

Beta Catenin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig and More (6)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 51067-2-AP

Synonyms

CTNNB, Catenin beta-1, Catenin beta 1, beta-Catenin, B-catenin


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Tested Applications

Positive WB detected inA431 cells, MCF-7 cells, human placenta tissue, mouse lung tissue, HeLa cells, SH-SY5Y cells, HEK-293 cells, NIH/3T3 cells, C6 cells, mouse liver tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue, human colon cancer tissue, mouse colon tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue
Positive IF/ICC detected inMCF-7 cells, HepG2 cells, HeLa cells, T-47D cells, mouse testis tissue
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

51067-2-AP targets Beta Catenin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat, pig, canine, chicken, zebrafish, hamster, sheep, goat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name catenin (cadherin-associated protein), beta 1, 88kDa
Calculated molecular weight 781 aa, 86 kDa
Observed molecular weight 92 kDa
GenBank accession numberBC058926
Gene symbol Beta Catenin
Gene ID (NCBI) 1499
RRIDAB_2086128
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin is a 92-kDa protein, originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)

Protocols

Product Specific Protocols
WB protocol for Beta Catenin antibody 51067-2-APDownload protocol
IHC protocol for Beta Catenin antibody 51067-2-APDownload protocol
IF protocol for Beta Catenin antibody 51067-2-APDownload protocol
IP protocol for Beta Catenin antibody 51067-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF,IP,IHC

Cell Metab

Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.

Authors - Xiuying Zhong
humanIF

Mol Cancer

PMID: 32727463

Authors - Cefan Zhou
humanWB

Hepatology

Basolateral CD147 induces hepatocyte polarity loss by E-cadherin ubiquitination and degradation in hepatocellular carcinoma progress.

Authors - Meng Lu
ratWB

Acta Pharm Sin B

Protocatechuic aldehyde protects cardiomycoytes against ischemic injury via regulation of nuclear pyruvate kinase M2.

Authors - Xunxun Wu
mouseWB

Hepatology

Long noncoding RNA DANCR increases stemness features of hepatocellular carcinoma via de-repression of CTNNB1.

Authors - Sheng-Xian Yuan
humanWB

Nat Commun

CD36 inhibits β-catenin/c-myc-mediated glycolysis through ubiquitination of GPC4 to repress colorectal tumorigenesis.

Authors - Yuan Fang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Udesh (Verified Customer) (08-16-2023)

Worked well for WB and IF

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:2000 for WB and 1:200 for IF
  • Cell Tissue Type: Bone Marrow MSCs
FH

Alzbeta (Verified Customer) (03-13-2019)

This antibody was used for IF staining of beta-catenin in primary neonatal rat cardiomyocyte culture. The antibody worked well after both ice cold-methanol or 4% PFA fixation, followed by the 3%BSA block. The incubation with primary (b-catenin) and secondary antibody (aRabbit-Alexa Fluor 488 Plus) lasted 1 hr at room temperature.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Primary rat neonatal cardiomyocytes
Beta Catenin Antibody Immunofluorescence, validation (1:100 dilution) in Primary rat neonatal cardiomyocytes (Cat no:51067-2-AP)