Validation Data Gallery

  • Structure of GFP-VHH.

    Structure of GFP-VHH.

ChromoTek anti-GFP recombinant VHH, 2x Cys for conjugation

Alpaca anti-GFP recombinant VHH, 1x Cys or 2x Cys contain 1 or 2 reactive Cysteines for Maleimide conjugation with a suitable reagent of your choice.
Cat No. gtCys2

Host/Type

Alpaca VHH

Reactivity

CFP, eGFP, wtGFP, GFP S65T, AcGFP, TagGFP, tagGFP2, sfGFP, pHluorin, eYFP, YFP, Venus, Citrine

Applications

Conjugation

Conjugate

Unconjugated

GFP VHH, GFP single domain antibody

Size/Concentration: 

-/ -


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Product Information

Alpaca anti-GFP recombinant VHH, 1x Cys or 2x Cys contain 1 or 2 reactive Cysteines for Maleimide conjugation with a suitable reagent of your choice.

DescriptionAlpaca anti-GFP recombinant VHH, 1x Cys or 2x Cys contain 1 or 2 reactive Cysteines for Maleimide conjugation with a suitable reagent of your choice.

• GFP-VHH:GFP complex is stable up to 80 °C, 1 mM DTT, 3 M Guanidinium•HCl, 8 M Urea, 2 M NaCl, 2 % Nonidet P40 Substitute, 1 % SDS, 1 % Triton X-100, 3 % Deoxycholate

• Fulfills highest requirements for antibody validation

• Structure and function are characterized

ApplicationsConjugation
ReactivityCFP, eGFP, wtGFP, GFP S65T, AcGFP, TagGFP, tagGFP2, sfGFP, pHluorin, eYFP, YFP, Venus, Citrine
ConjugateUnconjugated
TypeNanobody
ClassRecombinant
HostAlpaca
Affinity1 pM
Molecular Weight13.9 kDa
FormLiquid
RRIDAB_3665976
Storage Buffer10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, 0.09% sodium azide, 1mM TCEP
Storage ConditionStore at -20°C
ShippingDry ice
BackgroundGreen Fluorescent Proteins (GFPs) encompass a diverse range of proteins carrying a green chromophore, originating from various species and forming different protein lineages. Wildtype GFP consists of 238 amino acid residues (26.9 kDa). GFP was first identified in the jellyfish Aequorea victoria. It emits green light with a peak wavelength of 509 nm upon excitation by blue light at 395 nm.
This Nanobody is an alpaca recombinant VHH raised against GFP, reactive to a variety of GFP derivates, including CFP, eGFP, yeast eGFP, Clover, wtGFP, GFP S65T, AcGFP, TagGFP, tagGFP2, sfGFP, pHluorin, eYFP, YFP, Venus, YPET, Citrine and eCitrine and more.
For a full list of specificities see: Fluorescent protein specificity table