Validation Data Gallery
Product Information
Alpaca anti-GFP recombinant VHH, 1x Cys or 2x Cys contain 1 or 2 reactive Cysteines for Maleimide conjugation with a suitable reagent of your choice.
| Description | Alpaca anti-GFP recombinant VHH, 1x Cys or 2x Cys contain 1 or 2 reactive Cysteines for Maleimide conjugation with a suitable reagent of your choice. • GFP-VHH:GFP complex is stable up to 80 °C, 1 mM DTT, 3 M Guanidinium•HCl, 8 M Urea, 2 M NaCl, 2 % Nonidet P40 Substitute, 1 % SDS, 1 % Triton X-100, 3 % Deoxycholate • Fulfills highest requirements for antibody validation • Structure and function are characterized |
| Applications | Conjugation |
| Reactivity | CFP, eGFP, wtGFP, GFP S65T, AcGFP, TagGFP, tagGFP2, sfGFP, pHluorin, eYFP, YFP, Venus, Citrine |
| Conjugate | Unconjugated |
| Type | Nanobody |
| Class | Recombinant |
| Host | Alpaca |
| Affinity | 1 pM |
| Molecular Weight | 13.9 kDa |
| Form | Liquid |
| RRID | AB_3665976 |
| Storage Buffer | 10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, 0.09% sodium azide, 1mM TCEP |
| Storage Condition | Store at -20°C |
| Shipping | Dry ice |
| Background | Green Fluorescent Proteins (GFPs) encompass a diverse range of proteins carrying a green chromophore, originating from various species and forming different protein lineages. Wildtype GFP consists of 238 amino acid residues (26.9 kDa). GFP was first identified in the jellyfish Aequorea victoria. It emits green light with a peak wavelength of 509 nm upon excitation by blue light at 395 nm. This Nanobody is an alpaca recombinant VHH raised against GFP, reactive to a variety of GFP derivates, including CFP, eGFP, yeast eGFP, Clover, wtGFP, GFP S65T, AcGFP, TagGFP, tagGFP2, sfGFP, pHluorin, eYFP, YFP, Venus, YPET, Citrine and eCitrine and more. For a full list of specificities see: Fluorescent protein specificity table |
Documentation
| SDS |
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| gtCys2_SDS_GFP recombinant VHH, for 2x Cys conjugation (EN) |