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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using Zinc Alpha 2 Glycoprotein Recombinant monoclonal a (86781-3-RR)

    WB analysis of human plasma using 86781-3-RR

    human plasma were subjected to SDS PAGE followed by western blot with 86781-3-RR (AZGP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Zinc Alpha 2 Glycoprotein Recombinant monoclonal a (86781-3-RR)

    IHC staining of human breast cancer using 86781-3-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86781-3-RR (Zinc Alpha 2 Glycoprotein antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Zinc Alpha 2 Glycoprotein Recombinant monoclonal a (86781-3-RR)

    IHC staining of human breast cancer using 86781-3-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86781-3-RR (Zinc Alpha 2 Glycoprotein antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 86781-3-RR

    Affinity and Kinetic Characterization of 86781-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86781-3-RR against Human Zinc Alpha 2 Glycoprotein were performed. The affinity constant is 63.7 pM.

Zinc Alpha 2 Glycoprotein Recombinant monoclonal antibody

Zinc Alpha 2 Glycoprotein Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 86781-3-RR
Clone No.251605B9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

AZGP1, Zinc-alpha-2-glycoprotein, ZA2G, ZAG, Zn alpha 2 glycoprotein

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman plasma
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86781-3-RR targets Zinc Alpha 2 Glycoprotein in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg6736

Product name: Recombinant Human Zinc Alpha 2 Glycoprotein protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-N-rFc

Tag: N-rFc

Domain: 21-298 aa of BC033830

Sequence: QENQDGRYSLTYIYTGLSKHVEDVPAFQALGSLNDLQFFRYNSKDRKSQPMGLWRQVEGMEDWKQDSQLQKAREDIFMETLKDIVEYYNDSNGSHVLQGRFGCEIENNRSSGAFWKYYYDGKDYIEFNKEIPAWVPFDPAAQITKQKWEAEPVYVQRAKAYLEEECPATLRKYLKYSKNILDRQDPPSVVVTSHQAPGEKKKLKCLAYDFYPGKIDVHWTRAGEVQEPELRGDVLHNGNGTYQSWVVVAVPPQDTAPYSCHVQHSSLAQPLVVPWEAS

相同性解析による交差性が予測される生物種
Full Name alpha-2-glycoprotein 1, zinc-binding
Calculated molecular weight 298 aa, 34 kDa
Observed molecular weight 41 kDa
GenBank accession numberBC033830
Gene Symbol Zinc Alpha 2 Glycoprotein
Gene ID (NCBI) 563
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP25311
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Zinc-alpha-2-glycoprotein (AZGP1) is a 41-kDa soluble protein normally found in body fluids, functions as a lipid mobilizing factor (PMID: 19188554). It is known to be expressed in the secretory epithelia of the liver, lung, breast, GI tract and sweat glands, sharing significant structural similarity with the class I major histocompatibility complex (MHC) antigens (PMID: 3422450). AZGP1 is involved in carcinogenesis and differentiation. Altered expression of AZGP1 has been reported in breast cancer, prostate cancer and lung adenocarcinoma, hepatocellular carcinoma, pancreatic carcinoma and oral tumors (PMID: 22625427).

Protocols

Product Specific Protocols
IHC protocol for Zinc Alpha 2 Glycoprotein antibody 86781-3-RRDownload protocol
WB protocol for Zinc Alpha 2 Glycoprotein antibody 86781-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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