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ZWILCH Polyclonal antibody

ZWILCH Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14281-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Protein zwilch homolog, KNTC1AP, hZwilch

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inPC-3 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

14281-1-AP targets ZWILCH in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZWILCH fusion protein Ag5454 相同性解析による交差性が予測される生物種
Full Name Zwilch, kinetochore associated, homolog (Drosophila)
Calculated molecular weight 67 kDa
Observed molecular weight ~60 kDa
GenBank accession numberBC036900
Gene Symbol ZWILCH
Gene ID (NCBI) 55055
RRIDAB_2878040
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H900
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ZWILCH is a new component of the ZW10/ROD complex. The complex is a key player in the spindle checkpoint. ZWILCH has two isoforms with MW 67 kDa and 54 kDa.

Protocols

Product Specific Protocols
WB protocol for ZWILCH antibody 14281-1-APDownload protocol
IHC protocol for ZWILCH antibody 14281-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Nucleic Acids Res

ULK1 phosphorylates Mad1 to regulate spindle assembly checkpoint.

Authors - Fengjie Yuan
humanIHC

Biomedicines

The Enhanced Expression of ZWILCH Predicts Poor Survival of Adrenocortical Carcinoma Patients

Authors - Małgorzata Blatkiewicz
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