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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ZSCAN20 Polyclonal antibody

ZSCAN20 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20855-1-AP

Synonyms

KOX29, ZFP 31, Zinc finger protein 31, Zinc finger protein 360, Zinc finger protein KOX29, ZNF31, ZNF360, ZSCAN20

Validation Data Gallery

Applications:
  • WB analysis of HepG2 using 20855-1-AP

    WB analysis of HepG2 using 20855-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 20855-1-AP (ZSCAN20 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 20855-1-AP

    WB analysis of Jurkat using 20855-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20855-1-AP (ZSCAN20 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 20855-1-AP

    WB analysis of Jurkat using 20855-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20855-1-AP (ZSCAN20 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 20855-1-AP

    IHC staining of human small intestine using 20855-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20855-1-AP (ZSCAN20 Antibody) at dilution of 1:400.

  • IHC staining of human small intestine using 20855-1-AP

    IHC staining of human small intestine using 20855-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20855-1-AP (ZSCAN20 Antibody) at dilution of 1:200.

  • IHC staining of human small intestine using 20855-1-AP

    IHC staining of human small intestine using 20855-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20855-1-AP (ZSCAN20 Antibody) at dilution of 1:200.

  • IHC staining of human small intestine using 20855-1-AP

    IHC staining of human small intestine using 20855-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20855-1-AP (ZSCAN20 Antibody) at dilution of 1:400.

Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20855-1-AP is ZSCAN20 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZSCAN20 fusion protein Ag14798 相同性解析による交差性が予測される生物種
Full Name zinc finger and SCAN domain containing 20
Calculated molecular weight 1043 aa, 118 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC008827
Gene symbol ZSCAN20
Gene ID (NCBI) 7579
RRIDAB_2878753
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ZSCAN20 antibody 20855-1-APDownload protocol
IHC protocol for ZSCAN20 antibody 20855-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols