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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZSCAN2 Polyclonal antibody (32581-1-AP)

    WB analysis using 32581-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32581-1-AP (ZSCAN2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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ZSCAN2 Polyclonal antibody

ZSCAN2 Polyclonal Antibody for WB, ELISA
Cat No. 32581-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

ZNF854, Zinc finger protein 854, Zinc finger protein 29 homolog, Zinc finger and SCAN domain-containing protein 2, Zfp-29

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32581-1-AP targets ZSCAN2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZSCAN2 fusion protein Ag38126 相同性解析による交差性が予測される生物種
Full Name zinc finger and SCAN domain containing 2
Calculated molecular weight70 kDa
Observed molecular weight70 kDa
GenBank accession numberBC136342
Gene Symbol ZSCAN2
Gene ID (NCBI) 54993
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ7Z7L9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Zinc finger and SCAN domain-containing protein 2 (ZSCAN2, also known as ZFP29 and ZNF854) plays an important role in the maintenance of the SSC (spermatogonial stem cells) pool in vitro and is integral for robust regeneration of spermatogenesis in vivo following exposure to clastogens. Loss of ZSCAN2 expression alters key cellular processes in undifferentiated spermatogonia, such as translation, chromatin modification, and ubiquitin conjugation (PMID: 35244684).

Protocols

Product Specific Protocols
WB protocol for ZSCAN2 antibody 32581-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols