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Tested Applications
| Positive IP detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15835-1-AP targets ZPBP in IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ZPBP fusion protein Ag8585 相同性解析による交差性が予測される生物種 |
| Full Name | zona pellucida binding protein |
| Calculated molecular weight | 351 aa, 40 kDa |
| Observed molecular weight | 34 kDa |
| GenBank accession number | BC005223 |
| Gene Symbol | ZPBP |
| Gene ID (NCBI) | 11055 |
| RRID | AB_2878189 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BS86 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ZPBP (also known as ZPBP1), named for its binding to the oocyte zona pellucida, is a spermatid and spermatozoon protein that localizes to the acrosome. ZPBP may be implicated in gamete interaction during fertilization. It has been reported that absence of ZPBP1 prevents acrosome compaction and leads to acrosome fragmentation and sterility in male mice. (PMID: 17664285; 19204925)
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for ZPBP antibody 15835-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |