Validation Data Gallery
Tested Applications
| Positive WB detected in | HT-29 cells, HCT 116 cells, Caco-2 cells |
| Positive IP detected in | HT-29 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87266-1-RR targets ZO-3 in WB, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg5585 Product name: Recombinant human ZO3/Zonula occludens PROTEIN 3 protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 835-919 aa of NM_001267560.1 Sequence: DEPPAPALARSSEPVQADESQSPRDRGRISAHQGAQVDSRHPQGQWRQDSMRTYEREALKKKFMRVHDAESSDEDGYDWGPATDL 相同性解析による交差性が予測される生物種 |
| Full Name | tight junction protein 3 (zona occludens 3) |
| Calculated molecular weight | 101 kDa |
| Observed molecular weight | 140 kDa |
| GenBank accession number | NM_001267560.1 |
| Gene Symbol | ZO-3 |
| Gene ID (NCBI) | 27134 |
| RRID | AB_3745435 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O95049-1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Tight junction (or zonula occludens) forms the continuous intercellular barrier between epithelial and endothelial cells, which is required to separate tissue spaces and regulate selective movement of solutes across the epithelium and endothelium (PMID: 20066090). ZO-3 (also known as TJP3)is a key scaffold protein of tight junctions. ZO-3 belongs to a family of multidomain proteins known as the membrane-associated guanylate kinase homologs (MAGUKs).ZO-3 regulates the stability of Cyclin D1, thereby influencing the proliferation of epithelial cells (PMID: 21411630).
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for ZO-3 antibody 87266-1-RR | Download protocol |
| WB protocol for ZO-3 antibody 87266-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |