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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZNRF2-Specific Polyclonal antibody (20200-1-AP)

    WB analysis using 20200-1-AP (same clone as 20200-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 20200-1-AP (ZNRF2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20200-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using ZNRF2-Specific Polyclonal antibody (20200-1-AP)

    WB analysis of mouse kidney using 20200-1-AP (same clone as 20200-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20200-1-AP (ZNRF2-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20200-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using ZNRF2-Specific Polyclonal antibody (20200-1-AP)

    WB analysis of mouse brain using 20200-1-AP (same clone as 20200-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20200-1-AP (ZNRF2-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20200-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using ZNRF2-Specific Polyclonal antibody (20200-1-AP)

    WB analysis of mouse testis using 20200-1-AP (same clone as 20200-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 20200-1-AP (ZNRF2-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20200-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using ZNRF2-Specific Polyclonal antibody (20200-1-AP)

    WB analysis of mouse kidney using 20200-1-AP (same clone as 20200-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20200-1-AP (ZNRF2-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20200-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

ZNRF2-Specific Polyclonal antibody, PBS Only

ZNRF2-Specific Polyclonal Antibody for WB, Indirect ELISA
Cat No. 20200-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20200-1-PBS targets ZNRF2-Specific in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name zinc and ring finger 2
Calculated molecular weight 24 kDa
Observed molecular weight 30-35 kDa
GenBank accession numberNM_147128
Gene Symbol ZNRF2
Gene ID (NCBI) 223082
RRIDAB_2878657
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NHG8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ZNRF2 also named as RNF202, may play a role in the establisment and maintenance of neuronal transmission and plasticity via its ubiquitin ligase activity. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. The antibody is specific to ZNRF2.