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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZNHIT2 Polyclonal antibody (16885-1-AP)

    WB analysis using 16885-1-AP (same clone as 16885-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16885-1-AP (ZNHIT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16885-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using ZNHIT2 Polyclonal antibody (16885-1-AP)

    WB analysis of human brain using 16885-1-AP (same clone as 16885-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 16885-1-AP (ZNHIT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16885-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ZNHIT2 Polyclonal antibody (16885-1-AP)

    WB analysis using 16885-1-AP (same clone as 16885-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16885-1-AP (ZNHIT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16885-1-PBS in a different storage buffer formulation.

ZNHIT2 Polyclonal antibody, PBS Only

ZNHIT2 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 16885-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16885-1-PBS targets ZNHIT2 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag10351

Product name: Recombinant human ZNHIT2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-403 aa of BC052240

Sequence: MEPAGPCGFCPAGEVQPARYTCPRCNAPYCSLRCYRTHGTCAENFYRDQVLGELRGCSAPPSRLASALRRLRQQRETEDEPGEAGLSSGPAPGGLSGLWERLAPGEKAAFERLLSRGEAGRLLPPWRPWWWNRGAGPQLLEELDNAPGSDAAELELAPARTPPDSVKDASAAEPAAAERVLGDVPGACTPVVPTRIPAIVSLSRGPVSPLVRFQLPNVLFAYAHTLALYHGGDDALLSDFCATLLGVSGALGAQQVFASAEEALQAAAHVLEAGEHPPGPLGTRGAMHEVARILLGEGPTNQKGYTLAALGDLAQTLGRARKQAVAREERDHLYRARKKCQFLLAWTNENEAALTPLALDCARAHQAHAVVAEEVAALTGELERLWGGPVPPAPRTLIEELPS

相同性解析による交差性が予測される生物種
Full Name zinc finger, HIT type 2
Calculated molecular weight 403 aa, 43 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC052240
Gene Symbol ZNHIT2
Gene ID (NCBI) 741
RRIDAB_2220389
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UHR6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ZNHIT2 belongs to a family of HIT-finger proteins characterized by the presence of a HIT domain, a roughly 50-residue zinc-binding domain containing conserved cysteine (Cys) and histidine (His) residues. ZNHIT2 is a cofactor of R2TP/PFDL that targets the U5 snRNP. ZNHIT2 is highly expressed in testis.

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