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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZNHIT1 Polyclonal antibody (16595-1-AP)

    WB analysis using 16595-1-AP (same clone as 16595-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16595-1-AP (ZNHIT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16595-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using ZNHIT1 Polyclonal antibody (16595-1-AP)

    IHC staining of mouse liver using 16595-1-AP (same clone as 16595-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16595-1-AP (ZNHIT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16595-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using ZNHIT1 Polyclonal antibody (16595-1-AP)

    IHC staining of mouse liver using 16595-1-AP (same clone as 16595-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16595-1-AP (ZNHIT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16595-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using ZNHIT1 Polyclonal antibody (16595-1-AP)

    IF Staining of A431 using 16595-1-AP (same clone as 16595-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using ZNHIT1 antibody (16595-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16595-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ZNHIT1 Polyclonal antibody (16595-1-AP)

    IF Staining of HepG2 using 16595-1-AP (same clone as 16595-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZNHIT1 antibody (16595-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16595-1-PBS in a different storage buffer formulation.

ZNHIT1 Polyclonal antibody, PBS Only

ZNHIT1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 16595-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16595-1-PBS targets ZNHIT1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9741

Product name: Recombinant human ZNHIT1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-154 aa of BC017333

Sequence: MVEKKTSVRSQDPGQRRVLDRAARQRRINRQLEALENDNFQDDPHAGLPQLGKRLPQFDDDADTGKKKKKTRGDHFKLRFRKNFQALLEEQNLSVAEGPNYLTACAGPPSRPQRPFCAVCGFPSPYTCVSCGARYCTVRCLGTHQETRCLKWTV

相同性解析による交差性が予測される生物種
Full Name zinc finger, HIT type 1
Calculated molecular weight 154 aa, 18 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC017333
Gene Symbol ZNHIT1
Gene ID (NCBI) 10467
RRIDAB_2220382
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO43257
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ZNHIT1(Zinc finger HIT domain containing protein 1), aslo named CGBP1 (Cyclin G1 binding protein 1), is a subunit of the SNF-2 related CBP activator protein (SRCAP) complex, which can remodel chromatin by incorporating the histone variant H2A.Z into nucleosomes(PMID: 15647280). ZNHIT1 also can bind to myogenin promoter and mediate H2A.Z incorporation for its expression(PMID: 20473270). ZNHIT1 overexpression induces apoptosis and represses proliferation and invasion of breast cancer cells(PMID: 30928405).

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