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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using ZNF816 Polyclonal antibody (21221-1-AP)

    IHC staining of human ovary tumor using 21221-1-AP (same clone as 21221-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 21221-1-AP (ZNF816A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21221-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using ZNF816 Polyclonal antibody (21221-1-AP)

    IHC staining of human ovary tumor using 21221-1-AP (same clone as 21221-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 21221-1-AP (ZNF816A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21221-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using ZNF816 Polyclonal antibody (21221-1-AP)

    IF Staining of U-251 using 21221-1-AP (same clone as 21221-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using ZNF816A antibody (21221-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 21221-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using ZNF816 Polyclonal antibody (21221-1-AP)

    IF Staining of U-251 using 21221-1-AP (same clone as 21221-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using ZNF816A antibody (21221-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 21221-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using ZNF816 Polyclonal antibody (21221-1-AP)

    IF Staining of PC-3 using 21221-1-AP (same clone as 21221-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using ZNF816A antibody (21221-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 21221-1-PBS in a different storage buffer formulation.

ZNF816 Polyclonal antibody, PBS Only

ZNF816 Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 21221-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, Indirect ELISA

Synonyms

ZNF816A

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21221-1-PBS targets ZNF816 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag15658

Product name: Recombinant human ZNF816A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 399-506 aa of BC105930

Sequence: CEECDNVYIRRSHLERHRKIHTGEGSYKCKVCDKVFRSDSYLAEHQRVHTGEKPYKCNKCGRSFSRKSSLQYHHTLHTGEKPYTCNECGKVFSRRENLARHHRLHAGE

相同性解析による交差性が予測される生物種
Full Name zinc finger protein 816A
Calculated molecular weight 651 aa, 76 kDa
GenBank accession numberBC105930
Gene Symbol ZNF816A
Gene ID (NCBI) 125893
RRIDAB_3742021
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ0VGE8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ZNF816 ( Zinc finger protein 816), a direct target of ZBED4, was found to interact with multiple FDA-approved immunomodulatory agents, suggesting a potential ZBED4-ZNF816 regulatory axis with translational value.

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