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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZNF8 Polyclonal antibody (17448-1-AP)

    WB analysis using 17448-1-AP (same clone as 17448-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17448-1-AP (ZNF8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17448-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using ZNF8 Polyclonal antibody (17448-1-AP)

    IHC staining of mouse cerebellum using 17448-1-AP (same clone as 17448-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 17448-1-AP (ZNF8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17448-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using ZNF8 Polyclonal antibody (17448-1-AP)

    IF Staining of mouse cerebellum using 17448-1-AP (same clone as 17448-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using ZNF8 antibody (17448-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17448-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using ZNF8 Polyclonal antibody (17448-1-AP)

    IF Staining of mouse cerebellum using 17448-1-AP (same clone as 17448-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using ZNF8 antibody (17448-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17448-1-PBS in a different storage buffer formulation.

ZNF8 Polyclonal antibody, PBS Only

ZNF8 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 17448-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Zfp128, Zinc finger protein 8

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17448-1-PBS targets ZNF8 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag11571

Product name: Recombinant human ZNF8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 231-575 aa of BC039323

Sequence: NSDCHRDSSQAIPITELTKSQVQDKPYKCTDCGKSFNHNAHLTVHKRIHTGERPYMCKECGKAFSQNSSLVQHERIHTGDKPYKCAECGKSFCHSTHLTVHRRIHTGEKPYECQDCGRAFNQNSSLGRHKRTHTGEKPYTCSVCGKSFSRTTCLFLHLRTHTEERPYECNHCGKGFRHSSSLAQHQRKHAGEKPFECRQRLIFEQTPALTKHEWTEALGCDPPLSQDERTHRSDRPFKCNQCGKCFIQSSHLIRHQITHTREEQPHGRSRRREQSSSRNSHLVQHQHPNSRKSSAGGAKAGQPESRALALFDIQKIMQEKNPVHVIGVEEPSVGASMLFDIREST

相同性解析による交差性が予測される生物種
Full Name zinc finger protein 8
Calculated molecular weight 575 aa, 65 kDa
Observed molecular weight65 kDa
GenBank accession numberBC039323
Gene Symbol ZNF8
Gene ID (NCBI) 7554
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP17098
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ZNF8 is a transcriptional repressor. It may modulate BMP and TGF-beta signal transduction, through its interaction with SMAD proteins.

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