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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ZNF774 Polyclonal antibody

ZNF774 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 25449-1-AP

Synonyms

zinc finger protein 774, ZNF774

Validation Data Gallery

Applications:
  • WB analysis of HepG2 using 25449-1-AP

    WB analysis of HepG2 using 25449-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25449-1-AP (ZNF774 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 25449-1-AP

    IP experiment of HepG2 using 25449-1-AP

    IP result of anti-ZNF774 (IP:25449-1-AP, 3ug; Detection:25449-1-AP 1:600) with HepG2 cells lysate 2400ug.

Tested Applications

Positive WB detected inHepG2 cells
Positive IP detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25449-1-AP is ZNF774 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF774 fusion protein Ag22300 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 774
Calculated molecular weight 483 aa, 55 kDa
Observed molecular weight 55-63 kDa
GenBank accession numberBC067279
Gene symbol ZNF774
Gene ID (NCBI) 342132
RRIDAB_2880086
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ZNF774 antibody 25449-1-APDownload protocol
IP protocol for ZNF774 antibody 25449-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols