Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33035-1-AP targets ZNF768 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag37794 Product name: Recombinant human ZNF768 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-175 aa of BC013760 Sequence: MRSPEGYLRGNMSENEEEEISQQEGSGDYEVEEIPFGLEPQSPGFEPQSPEFEPQSPRFEPESPGFESRSPGLVPPSPEFAPRSPESDSQSPEFESQSPRYEPQSPGYEPRSPGYEPRSPGYESESSRYESQNTELKTQSPEFEAQSSKFQEGAEMLLNPDEKSPLNISVGVHPL 相同性解析による交差性が予測される生物種 |
| Full Name | zinc finger protein 768 |
| Calculated molecular weight | 60 kDa |
| Observed molecular weight | 70 kDa |
| GenBank accession number | BC013760 |
| Gene Symbol | ZNF768 |
| Gene ID (NCBI) | 79724 |
| RRID | AB_3742829 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9H5H4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Zinc finger protein 768 (ZNF768) is a zinc finger protein that is crucial in linking oncogenic RAS signaling to cellular senescence and proliferation control. It is a transcription factor that associates with euchromatin and promoter regions of many genes in a cell type-specific manner. ZNF768 is required for cell growth and survival, and its depletion induces a strong proliferative and pro-apoptotic response in several cell lines in vitro. ZNF768 is also involved in regulating cell cycle effectors and p53 targets, with its overexpression sufficient to bypass RAS-induced senescence in a p53-dependent manner (PMID: 34404770; 35419475).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZNF768 antibody 33035-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |