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Validation Data Gallery

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  • Western Blot (WB) analysis of RAW 264.7 cells using ZNF75D Polyclonal antibody (21583-1-AP)

    WB analysis of RAW 264.7 using 21583-1-AP

    WB result of ZNF75D antibody (21583-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZNF75D transfected RAW 264.7 cells.

  • Western Blot (WB) analysis of various lysates using ZNF75D Polyclonal antibody (21583-1-AP)

    WB analysis using 21583-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21583-1-AP (ZNF75D antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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ZNF75D Polyclonal antibody

ZNF75D Polyclonal Antibody for WB, ELISA
Cat No. 21583-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

D8C6, Zinc finger protein 75, zinc finger protein 75D, Zinc finger protein 82, ZNF75, ZNF75D, ZNF82

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21583-1-AP targets ZNF75D in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF75D fusion protein Ag16139 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 75D
Calculated molecular weight 510 aa, 59 kDa
Observed molecular weight35 kDa
GenBank accession numberBC109100
Gene Symbol ZNF75D
Gene ID (NCBI) 7626
RRIDAB_3085661
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP51815
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ZNF75D (zinc finger protein 75D), also known as ZNF75. It is expected to be located in nucleus and ubiquitous expression in prostate and adrenal. The calculated molecular weight of METAP1 is 60 kDa. The gene encodes a protein that likely functions as a transcription factor. ZNF75D belongs to the ZNF75 family, includes an N-terminal SCAN domain, a KRAB box, and five C2H2-type zinc finger motifs. Another functional gene belonging to this family is located on chromosome 16, while pseudogenes have been identified on chromosomes 11 and 12 (PMID: 8661144). It may be involved in transcriptional regulation.

Protocols

Product Specific Protocols
WB protocol for ZNF75D antibody 21583-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols