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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ZNF689 Polyclonal antibody (25573-1-AP)

    WB analysis of HeLa using 25573-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25573-1-AP (ZNF689 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of HepG2 cells using ZNF689 Polyclonal antibody (25573-1-AP)

    WB analysis of HepG2 using 25573-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25573-1-AP (ZNF689 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of mouse testis tissue using ZNF689 Polyclonal antibody (25573-1-AP)

    WB analysis of mouse testis using 25573-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25573-1-AP (ZNF689 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of mouse testis tissue using ZNF689 Polyclonal antibody (25573-1-AP)

    WB analysis of mouse testis using 25573-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25573-1-AP (ZNF689 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

ZNF689 Polyclonal antibody

ZNF689 Polyclonal Antibody for WB, ELISA
Cat No. 25573-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

TIPUH1, zinc finger protein 689, ZNF689

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, mouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25573-1-AP targets ZNF689 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag22252

Product name: Recombinant human ZNF689 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 77-173 aa of BC014000

Sequence: LISWLERNTDDWEPAALDPQEYPRGLTVQRKSRTRKKNGEKEVFPPKEAPRKGKRGRRPSKPRLIPRQTSGGPICPDCGCTFPDHQALESHKCAQNL

相同性解析による交差性が予測される生物種
Full Name zinc finger protein 689
Calculated molecular weight 500 aa, 57 kDa
Observed molecular weight 50-57 kDa
GenBank accession numberBC014000
Gene Symbol ZNF689
Gene ID (NCBI) 115509
RRIDAB_2880134
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96CS4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ZNF689 antibody 25573-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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