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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZNF683 Polyclonal antibody (32229-1-AP)

    WB analysis using 32229-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32229-1-AP (ZNF683 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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ZNF683 Polyclonal antibody

ZNF683 Polyclonal Antibody for WB, ELISA
Cat No. 32229-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Zinc finger protein 683, Tissue-resident T-cell transcription regulator protein ZNF683, Homolog of Blimp-1 in T-cell, Hobit

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, NK-92 cells, RAW 264.7 cells, Raji cells, mouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32229-1-AP targets ZNF683 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF683 fusion protein Ag37412 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 683
Calculated molecular weight57 kDa
Observed molecular weight57 kDa
GenBank accession numberBC028731
Gene Symbol ZNF683
Gene ID (NCBI) 257101
RRIDAB_3670226
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ8IZ20
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Zinc finger protein 683 (ZNF683, also known as Hobit) is a transcription factor that mediates a transcriptional program in various innate and adaptive immune tissue-resident lymphocyte T-cell types such as tissue-resident memory T (Trm) and natural killer cells (PMID: 36869093; 28555134; 36245253). It probably is a cancer-specific Trms marker (PMID: 36869093).

Protocols

Product Specific Protocols
WB protocol for ZNF683 antibody 32229-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols