Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, NK-92 cells, RAW 264.7 cells, Raji cells, mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32229-1-AP targets ZNF683 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag37412 Product name: Recombinant human ZNF683 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-296 aa of BC028731 Sequence: MALGGTGGSLSPSLDFQLFRGDQVFSACRPLPDMVDAHGPSCASWLCPLPLAPGRSALLACLQDLDLNLCTPQPAPLGTDLQGLQEDALSMKHEPPGLQASSTDDKKFTVKYPQNKDKLGKQPERAGEGAPCPAFSSHNSSSPPPLQNRKSPSPLAFCPCPPVNSISKELPFLLHAFYPGYPLLLPPPHLFTYGALPSDQCPHLLMLPQDPSYPTMAMPSLLMMVNELGHPSARWETLLPYPGAFQASGQALPSQARNPGAGAAPTDSPGLERGGMASPAKRVPLSSQTGTAALPY 相同性解析による交差性が予測される生物種 |
| Full Name | zinc finger protein 683 |
| Calculated molecular weight | 57 kDa |
| Observed molecular weight | 57 kDa |
| GenBank accession number | BC028731 |
| Gene Symbol | ZNF683 |
| Gene ID (NCBI) | 257101 |
| RRID | AB_3670226 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q8IZ20 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Zinc finger protein 683 (ZNF683, also known as Hobit) is a transcription factor that mediates a transcriptional program in various innate and adaptive immune tissue-resident lymphocyte T-cell types such as tissue-resident memory T (Trm) and natural killer cells (PMID: 36869093; 28555134; 36245253). It probably is a cancer-specific Trms marker (PMID: 36869093).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZNF683 antibody 32229-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |