Validation Data Gallery
Tested Applications
| Positive WB detected in | human plasma sample |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27590-1-AP targets ZNF653 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag23904 Product name: Recombinant human ZNF653 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC014187 Sequence: MAERALEPEAEAEAEAGAGGEAAAEEGAAGRKARGRPRLTESDRARRRLESRKKYDVRRVYLGEAHGPWVDLRRRSGWSDAKLAAYLISLERGQRSGRHGKPWEQVPKKPKRKKRRRRNVNCLKNVVIWYEDHKHRCPYEPHLAELDPTFGLYTTAVWQCEAGHRYFQDLHSPLKPLSDSDPDSDKVGNGLVAGSSDSSSSGSASDSEESPEGQPVKAAAAAAAATPTSPVGSSGLITQEGVHIPFDVHHVESLAEQGTPLCSNPAGNGPEALETVVCVPVPVQVGAGPSALFENVPQEALGEVVASCPMPGMVPGSQVIIIAGPGYDALTAEGIHLNMAAGSGVPGSGL 相同性解析による交差性が予測される生物種 |
| Full Name | zinc finger protein 653 |
| Observed molecular weight | 67 kDa |
| GenBank accession number | BC014187 |
| Gene Symbol | ZNF653 |
| Gene ID (NCBI) | 115950 |
| RRID | AB_3669613 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96CK0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ZNF653, also named ZIP67, belongs to the krueppel C2H2-type zinc-finger protein family. ZNF653 is a transcriptional repressor, which may repress NR5A1, PPARG, NR1H3, NR4A2, ESR1, and NR3C1 transcriptional activity. ZNF653 is highly expressed in testis, cerebellum, temporal lobe, hippocampus and adrenal gland, and moderately expressed in spleen, uterus, thymus, pancreas, kidney, stomach and rectum. The calculated molecular weight of ZNF653 is 67 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ZNF653 antibody 27590-1-AP | Download protocol |
| WB protocol for ZNF653 antibody 27590-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |