Validation Data Gallery
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Tested Applications
| Positive WB detected in | Y79 cells, Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25956-1-AP targets ZNF644 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ZNF644 fusion protein Ag22798 相同性解析による交差性が予測される生物種 |
| Full Name | zinc finger protein 644 |
| Calculated molecular weight | 1327 aa, 150 kDa |
| Observed molecular weight | 175 kDa |
| GenBank accession number | BC063683 |
| Gene Symbol | ZNF644 |
| Gene ID (NCBI) | 84146 |
| RRID | AB_3669512 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H582 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
G9a/GLP complex is associated with Polycomb Repressive Complex 2 (PRC2).The G9a/GLP complex also regulates DNA methylation during early embryogenesis, which is independent of their methyltransferase activity. ZNF644 and WIZ, two zinc finger proteins, interact with G9a and GLP, respectively. These two zinc finger proteins target G9a and GLP to genomic loci for the regulation of gene transcription.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZNF644 antibody 25956-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |