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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ZNF639 Polyclonal antibody (25817-1-AP)

    WB analysis of HeLa using 25817-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25817-1-AP (ZNF639 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using ZNF639 Polyclonal antibody (25817-1-AP)

    IP experiment of HeLa using 25817-1-AP

    IP result of anti-ZNF639 (IP:25817-1-AP, 4ug; Detection:25817-1-AP 1:300) with HeLa cells lysate 3960ug.

ZNF639 Polyclonal antibody

ZNF639 Polyclonal Antibody for WB, IP, ELISA
Cat No. 25817-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

6230400O18Rik, ANC 2H01, ANC_2H01, ZASC1, zinc finger protein 639, Zinc finger protein ANC_2H01, Zinc finger protein ZASC1, ZNF639

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25817-1-AP targets ZNF639 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag19854

Product name: Recombinant human ZNF639 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 136-485 aa of BC026181

Sequence: EDVPIAVEVHAISEDYDIETENNSSESLQDQTDEEPPAKLCKILDKSQALNVTAQQKWPLLRANSSGLYKCELCEFNSKYFSDLKQHMILKHKRTDSNVCRVCKESFSTNMLLIEHAKLHEEDPYICKYCDYKTVIFENLSQHIADTHFSDHLYWCEQCDVQFSSSSELYLHFQEHSCDEQYLCQFCEHETNDPEDLHSHVVNEHACKLIELSDKYNNGEHGQYSLLSKITFDKCKNFFVCQVCGFRSRLHTNVNRHVAIEHTKIFPHVCDDCGKGFSSMLEYCKHLNSHLSEGIYLCQYCEYSTGQIEDLKIHLDFKHSADLPHKCSDCLMRFGNERELISHLPVHETT

相同性解析による交差性が予測される生物種
Full Name zinc finger protein 639
Calculated molecular weight 485 aa, 56 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC026181
Gene Symbol ZNF639
Gene ID (NCBI) 51193
RRIDAB_2880251
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UID6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ZNF639, also named as ZASC1, is a 485 amino acid protein, which locates in the nucleus and contains 8 C2H2-type zinc fingers and belongs to the krueppel C2H2-type zinc-finger protein family. ZNF639 binds DNA and may function as a transcriptional repressor.

Protocols

Product Specific Protocols
IP protocol for ZNF639 antibody 25817-1-APDownload protocol
WB protocol for ZNF639 antibody 25817-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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