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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZNF618 Polyclonal antibody (31972-1-AP)

    WB analysis using 31972-1-AP (same clone as 31972-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31972-1-AP (ZNF618 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31972-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using ZNF618 Polyclonal antibody (31972-1-AP)

    WB analysis of SH-SY5Y using 31972-1-AP (same clone as 31972-1-PBS)

    WB result of ZNF618 antibody (31972-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZNF618 transfected SH-SY5Y cells. This data was developed using the same antibody clone with 31972-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using ZNF618 Polyclonal antibody (31972-1-AP)

    IHC staining of human cervical cancer using 31972-1-AP (same clone as 31972-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 31972-1-AP (ZNF618 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31972-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using ZNF618 Polyclonal antibody (31972-1-AP)

    IHC staining of human stomach cancer using 31972-1-AP (same clone as 31972-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 31972-1-AP (ZNF618 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31972-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using ZNF618 Polyclonal antibody (31972-1-AP)

    IF Staining of A549 using 31972-1-AP (same clone as 31972-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ZNF618 antibody (31972-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 31972-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ZNF618 Polyclonal antibody, PBS Only

ZNF618 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 31972-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

KIAA1952, Zinc finger protein 618

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31972-1-PBS targets ZNF618 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag36487

Product name: Recombinant human ZNF618 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 200-382 aa of NM_001318042.1

Sequence: YSCFQEHRDLHAVDVFSVEGAPENRADPFDQGVVATDEVKEEPPEPFQKIGPKTGNYTCEFCGKQYKYYTPYQEHVALHAPISTAPGWEPPDDPDTGSECSHPEVSPSPRFVAAKTQTNQSGKKAPASVVRCATLLHRTPPATQTQTFRTPNSGSPASKATAAESAFSRRVEGKAQNHFEETN

相同性解析による交差性が予測される生物種
Full Name zinc finger protein 618
Calculated molecular weight105kDa，954aa
Observed molecular weight100-113 kDa
GenBank accession numberNM_001318042.1
Gene Symbol ZNF618
Gene ID (NCBI) 114991
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ5T7W0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Zinc finger protein 618 (ZNF618) is a key protein associated with DNA methylation and acts as a specific 5hmC reader in vivo to regulate UHRF2 function by promoting UHRF2 chromatin localization. In addition, ZNF618 is not only associated with the induction, maintenance or progression of female breast cancer, but is also up-regulated in lung cancer cells.

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